Arch food sci technol | Volume 1, Issue 1 | Research Article | Open Access
Afsaneh Mollahosseini1*, Farid Mostafaei1, Ali Khadir2, Shahrzad Javanshir1 and Elham Pourian1
1Department of Chemistry, Iran University of Science and Technology, Iran
2Department of Civil and Environmental Engineering, Iran University of Science and Technology, Iran
*Correspondance to: Afsaneh Mollahosseini
Fulltext PDFThe present study focused on the separation of lysozyme from various media using adsorption technology. Firstly, Fe3O4/isinglass nanocomposite was synthesized and then characterized by Fourier Transform Infrared Spectrometer (FTIR), Scanning Electron Microscope (SEM), Transmission Electron Microscopy (TEM) and Vibrating Sample Magnetometer (VSM). The maximum adsorbent capacity was achieved at a contact time of 90 min, pH of 6.2, the initial LYZ concentration of 0.5 mg ml-1, and the adsorbent dosage of 15 mg. The obtained experimental data suited well with the Langmuir isotherm model by prediction the maximum adsorption capacity and a correlation coefficient of 149.25 mg/g and 0.9532, respectively. The kinetic data were well fitted using a pseudo second order kinetic model. Under optimized condition, the relative standard deviation with three replicates was 3.2%. The mixture of lysozyme and bovine serum albumin was used as a mixture model protein to investigate the adsorption selectivity. The magnetite/isinglass composite was then used for the extraction of lysozyme from egg white solutions. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) results showed that adsorption of lysozyme from protein mixture is selective and extracted lysozyme from egg white showed high purity.
Protein separation; Lysozyme; Egg white; Fe3O4@IG nanoparticles; Adsorption
Mollahosseini A, Mostafaei F, Khadir A, Javanshir S, Pourian E. Effective Adsorption and Separation of Lysozyme with Magnetite/Isinglass Composite Nanoparticles: Investigation of the Operational Parameters. Arch Food Sci Technol. 2020; 1(1): 1003..