Mengmeng Lu1,2, Yiyan Wang1, Xihe Li3 and Wenzhong Zhou1*
1Department of Basic Veterinary Medicine, China Agricultural University, China
2School of Life Sciences, Henan University, China
3Research Center for Animal Genetic Resources of Mongolian Plateau, Inner Mongolia University, China
Surface charge (zeta potential) is one of the most important factors of drug nanocarriers. In this study, Tilmicosin-loaded Solid Lipid Nanoparticles (TMS-SLNs) were prepared by a hot homogenization and ultra-sonication technique using castor oil as lipid matrix and polyvinyl alcohol as surfactant. Dimethyl Dioctadecyl Ammonium Chloride (DDAC, 2%, w/w) was used as a cationic surfactant to modify the zeta potential of the SLNs for the purpose of improving the formulation. The obtained TMS-SLNs were characterized and the antibacterial ability was evaluated in vitro and in vivo against avian pasteurella. The results showed that addition of DDAC switched the zeta potential from - 11.7 ± 1.5 mv to 38.9 ± 1.5 mv. DDAC significantly increased the drug loading and enhanced the sustained release property of the TMS-SLNs. Scanning electron microscopy showed the SLNs were spherical and DDAC made no change of the morphology. Fluorescent microscopy revealed that DDAC enhanced the nanoparticle binding to the bacteria. The modified TMS-SLNs showed enhanced in vitro and in vivo antibacterial efficacy and good stability similar to the unmodified formulation. These results suggest that DDAC modification facilitated drug entrapment and sustained release property of the TMS-SLNs. This study presents useful information towards the design of TMS-SLN formulations.
Tilmicosin; Solid lipid nanoparticle; Surface charge; Avian Pasteurella; Antibacterial efficacy
Lu M, Wang Y, Li X, Zhou W. Surface Charge Modification and Antibacterial Study on Tilmicosin-Loaded Solid Lipid Nanoparticles against Avian Pasteurella. Ann Pharmacol Pharm. 2021;6(1):1198..