Ann Pharmacol Pharm | Volume 2, Issue 6 | Review Article | Open Access

Genotoxicity of Selected Chinese Medicinal Plants, Elephantopus Scaber, Glycyrrhiza Uralensis and Salvia Miltiorrhiza on Allium Cepa Assay

Kwan Yuet Ping1, Shamarina Shohaimi1, Sreenivasan Sasidharan2* and Umi Kalsom Yusuf1

1Department of Biology, Universiti Putra Malaysia, Malaysia
2Department of Molecular Medicine, Universiti Sains Malaysia, Malaysia

*Correspondance to: Sreenivasan Sasidharan 

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Abstract

The genotoxic effects of leaf and root extracts of Elephantopus scaber, root extracts of Salvia miltiorrhiza and Glycyrrhiza uralensis on the mitotic cells in Allium cepa root tips were studied. The crude extracts of 1 μg/ml, 50 μg/ml, 500 μg/ml and 1000 μg/ml were tested on root meristems of A. cepa. Quercetin was used as positive control and distilled water as negative control. The result showed that mitotic index decreased as the concentrations of crude extracts increased. The increase of the genotoxic effect corresponds to a decrease of mitotic activity. A dose-dependent increase of chromosome aberrations was observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Result of this study confirmed that the methanol leaf extracts of E.scaber exerted significant genotoxic effects followed by methanol root extracts of S. miltiorrhiza at 1000 μg/ml respectively.

Keywords:

Genotoxicity; Allium cepa; Quercetin; Mitotic index; Chromosome aberrations

Citation:

Ping KY, Shohaimi S, Sasidharan S, Yusuf UK. Genotoxicity of Selected Chinese Medicinal Plants, Elephantopus Scaber, Glycyrrhiza Uralensis and Salvia Miltiorrhiza on Allium Cepa Assay. Ann Pharmacol Pharm. 2017; 2(13): 1070.

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