Ann Pharmacol Pharm | Volume 2, Issue 6 | Review Article | Open Access

Simple and Rapid Separation and Determination of Phospholipids by HPLC-UV System

Shafiq-ur-Rehman*, Shaheen Rehman, D Welter, D Wildenauer and M Ackenheil

Department of Neurochemistry, Institute of Psychiatry, Nussbaum Strasse, Germany

*Correspondance to: Shafiq-ur-Rehman 

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Abstract

Phospholipids are important class of complex molecules that provide structural and functional roles in the biological systems. Phospholipids anomalies and disorders are generalized membrane defects and so can be detected in a variety of tissues, including body fluids and blood elements. Highperformance liquid chromatographic method for the analysis of major phospholipids is reported. Isocratic separation of phospholipids was achieved on silica gel column using elution solvent mixture of acetonitrile-methanol-phosphoric acid (100:10:1.8. v/v) within 9.5 min. Detection of phospholipids was attained by UV spectrometer at 203 nm with detection limit ~5 ng. The present method of HPLC hyphenated-UV system offers advantages of high speed and simplicity for the separation and detection of a variety of phospholipids including phosphatidylserine, lysophosphatidylserine, phosphatidylcholine , lysophosphatidylcholine, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidic acid and sphingomyelin in routine clinical studies pertaining to membrane/ cell disorders in health, toxicity, diseases, and biological systems, and in basic research, as well.

Keywords:

Phospholipids analysis; High-performance liquid chromatography; HPLC-UV; Isocratic method

Citation:

Shafiq-ur-Rehman, Shaheen Rehman, D Welter, D Wildenauer, M Ackenheil. Simple and Rapid Separation and Determination of Phospholipids by HPLC-UV System. Ann Pharmacol Pharm. 2017; 2(6): 1063.

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