Ann Pharmacol Pharm | Volume 2, Issue 4 | Research Article | Open Access
Gaurav Upadhyay1*, Jai Malik2, Rohit Joshi3, Lakshmayya4 and UK Singh1
1Kharvel Subharti College of Pharmacy, Swami Veevekananda Subharti University, Subhartipuram, Delhi-Haridwar by pass, Meerut (U.P., India)
2Department of Pharmacognosy and Herbal Drug Technology, Indo-Soviet Friendship College of Pharmacy, Ferozpur Road, Moga (Punjab), India
3Research Scientist (Stress Physiology and Molecular Biology Laboratory) School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India
4Department of Pharmacy,GRD (PG) IMT, 214, Rajpur Road, Dehradun, Uttarakhand
*Correspondance to: Gaurav Upadhyay
Fulltext PDFRoylea elegans (Lamiaceae) is well known traditional plant of Garhwal and Kumaon regions of Uttarakhand, India used to treat jaundice and liver disorder. The present study was designed to evaluate its hepatoprotective effect, and to validate its traditional claims. Hepatoprotective activity of lyophilized hydro alcoholic extract of aerial parts of Roylea elegans (HAE) was extracted using carbon tetrachloride (CCL4) and paracetamol (PCM) induced Hepatotoxicity at a dose level of 1 ml/kg, s.c. and 3 ml/kg, p.o, respectively. Different parameters were used for estimation of hepatic injury. Blood serum parameter as serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphate (ALP), and total billirubin (TB) was evaluated for Hepatotoxicity and tissue parameters as thio-barbituric acid reactive substances (TBARS) and reduced glutathione (GSH) levels was evaluated on oxidative stress for protective effects of plant at a different dose levels (100, 200 and 400 mg/kg). The biochemical observations were also supplemented by histopathological examination. The preliminary phytochemical sceening of HAE showed the presence of alkaloids, triterpenes and carbohydrates. The HAE extract (100, 200 and 400 mg/kg) significantly (p<0.05) prevented the increase levels of serum enzymes for both CCL4 and PCM induced Hepatotoxicity. Furthermore, the lyophilized HAE also exhibited antioxidant activity at above dose levels significantly (p<0.05) decreases the TBARS levels and increases the GSH levels against both the models, also supported by histopathological study.
Conclusion: The present investigation clearly indicates the hepatoprotective effect of Roylea elegans Wall. May be attributed to antioxidant potential of phytoconstituents.
Roylea elegans; Histopathology; Hepatoprotective; Biochemical estimation; Lyophillized; Hepatotoxicity
Upadhyay G, Malik J, Joshi R, Lakshmayya, Singh UK. Hepatoprotective Potential of Lyophilized Hydro-Alcoholic Extract of Roylea Elegans Wall. Against CCL4 and PCM Induced Hepatotoxicity in Wistar Rats. Ann Pharmacol Pharm. 2017; 2(4): 1045.