Ann Pharmacol Pharm | Volume 2, Issue 4 | Short Communication | Open Access

Co-culturing of Marine and Terrestrial Actinomycetesto Obtain Novel Secondary Metabolites

Lokesh Ravi¹, Baskar R², Sarveswari S² and Kannabiran Krishnan^1*

¹Department of Biomedical Sciences, VIT University, India
²Department of Chemistry, VIT University, India

^*Correspondance to: K Kannabiran 

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Abstract

Co-culturing of actinomycetes is a classical approach used to enhance bioactivity. In this study, co-culturing of terrestrial and marine actinomycetes was performed to obtain increased anticancer cytotoxic activity. Crude extract prepared from co-cultured isolates showed increased anticancer activity. Although normal and healthy actinomycetes filament was observed during co-culture, spore formation was suppressed greatly by co-culturing. Crude extract of co-culture isolates demonstrated the IC₅₀ of 20 μg/ml, while the monoculture of isolates showed the IC₅₀ of 40 μg/ml. Both the isolate were greatly diverse from each other, morphologically and biochemically. To the best of our knowledge, this is the first report on co-culture of marine and terrestrial actinomycetes enhance the bioactivity. During co culturing organisms compete each other for their survival rather individually. However, further studies are required to identify the variation in the composition of secondary metabolites produced by them during co-culturing.

Citation:

Ravi L, Baskar R, Sarveswari S, Krishnan K. Co-culturing of Marine and Terrestrial Actinomycetesto Obtain Novel Secondary Metabolites. Ann Pharmacol Pharm. 2017; 2(4): 1041.