Shahira H EL Moslamy*
Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, EgyptFulltext PDF
A major importance features that could be considered during fed-batch fermentation strategy are gaseous and substrate transfer processes, inoculums generation, viscosity, pH, temperature, and all other pathways that made any biochemical, genetic, and morphological changes. Since there are, some products associated with microbial growth and other non-associated products. So a linear relationship between the tested parameter and the final impact should be evaluated through fedbatch fermentation processes. Ideally, the successful scaling-up fermentation system would mean a higher yield product by using low-cost culturing, and low-energy requirements. Since the scalingup of microbial cells via batch fermentation system is complicated and unpredictable process due to the fluid dynamic generated stresses. The fed-batch system provides a very dynamic environment with large spatial and temporal heterogeneities that can induce multiple physiological responses in microbial cells, depending on the mathematical model of the feeding regime. A maximum specific growth rate is a critical factor indicating the development of any fed-batch fermentation program. Physiological characters of microbial cells are the mandatory step of any development. Since the specific growth rate is a critical parameter restricting maximum possible feed addition in order to avoid substrate accumulations. Such systematic development of fed-batch processes would allow an early assessment of product kinetics, as well as avoiding unnecessary additional cultivations and variations in the final results. By using fed-batch fermentation modes, most of the industrially important bioactive metabolites are produced at optimal or sub-optimal operations conditions. There are numerous examples that can be explained the applications of an optimal fed-batch process were listed in this review.
Shahira H EL Moslamy. Application of Fed-Batch Fermentation Modes for Industrial Bioprocess Development of Microbial Behaviour. Ann Biotechnol Bioeng. 2019; 1(1): 1001.