Am J Clin Microbiol Antimicrob | Volume 3, Issue 1 | Review Article | Open Access

Molecular Rapid Diagnostic Method for Nosocomial Non- Fermenting Gram-Negative Bacilli

Emami A*, Pirbonyeh N and Javanmardi F

Department of Microbiology, Burn & Wound Healing Research Center, Iran

*Correspondance to: Emami A 

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For appropriate antibiotic therapy and avoid unnecessary use of drugs, accurate diagnosis of
Nosocomial Infections (NI) is crucial. There are three Gram-negative bacteria in NI’s; P. aeruginosa,
A. baumannii and S. maltophilia, which may misdiagnosed with together in traditional diagnostic
methods. Due to this, a rapid molecular diagnosis kit has been designed in Shiraz Burn Research
Center. According to the sensitivity and specificity percent analyses results, following results were
obtained respectively: P. aeruginosa: 100%, 96.8%; A. baumannii: 100%, 100% and S. maltophilia:
96.7%, and 93.8%. Based on these results this multiplex-PCR kit with three primer sequences is
responsible for timely and appropriate detection of studied bacteria.


Pseudomonas aeruginosa; Acinetobacter baumannii; Stenotrophomonas maltophilia;
Nosocomial Infection


Emami A, Pirbonyeh N, Javanmardi F. Molecular Rapid Diagnostic Method for Nosocomial Non-Fermenting Gram- Negative Bacilli. Am J Clin Microbiol Antimicrob. 2020; 3(1): 1047.

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