Rishikesan Rathnasamy1, Ranjith Pakkath Karuvalam2, Rajeesh Pakkath2, Prabakaran Kamalakannan3 and Arvind Sivasubramanian4*
1Department of Analytical Chemistry, PG and Research Centre, Sri Paramakalyani College, India
2School of Chemical sciences, Kannur University, India
3Department of Organic Chemistry, School of Advanced Sciences, VIT University, India
4Department of Chemistry, SASTRA University, India
An Accurate, Precise, Rugged, Reproducible Reverse Phase High Pressure Liquid Chromatographic (RP-HPLC) method has been developed and validated for the simultaneous estimation of Lopinavir and Ritonavir in pharmaceutical dosage form of anti-retroviral protease inhibitor class of compounds. The method was achieved by using the mixed organic mobile phases of Acetonitrile: Methanol with the buffer of potassium dihydrogen phosphate (pH 3.5) and Xbridge C18 (250 mm × 4.6 mm i.d., 3.5 μm. The flow rate was set as 1.1 ml/min has provided a good peak shape of Ritonavir and Lopinavir were found well separated at 5.7 and 6.6 min respectively.
Lopinavir; Ritonavir; Xbridge C18
Rathnasamy R, Karuvalam RP, Pakkath R, Kamalakannan P, Sivasubramanian A. RP-HPLC Method Development and Method Validation of Lopinavir and Ritonavir in Pharmaceutical Dosage Form. Am J Clin Microbiol Antimicrob. 2018; 1(1): 1002.