Bushra Zahoor1, Aqeel Javeed1*, Naeem ur Rehman2, Ahmed Tokhi2, Zainab Ahmed2, Khadijeh Esmaeilpour3*, Muhammad Ashraf1, Jawad Nazir1 and Ahmad Hamdani D1
1Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Pakistan
2Department of Pharmacy, COMSATS University Islamabad, Pakistan
3Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Iran
Introduction: Piroxicam has analgesic, anti-inflammatory and antipyretic activity. It inhibits the formation of prostaglandins, important regulator of the immune system. The current study was conducted to assess immunomodulatory effects of piroxicam on cell mediated as well as humoral immunity in mice. Material and method: Type 4 Delayed Hypersensitivity reaction (TDH) assay along with cyclophosphamide induced neutropenia were performed for the study of cell mediated immunity. The effect of the drug on humoral immunity was studied by the Hemagglutination Assay (HA) and mice lethality test. Mice were treated with piroxicam at a dose 5 mg/kg and 10 mg/kg intraperitoneally. Result: The data was evaluated and analyzed via ANOVA (*P<0.05). There was significant reduction in mean skin thickness and neutrophil count in DTH assay and cyclophosphamide induced neutropenia respectively when compared to the control group. The dose dependent reduction was observed in HA assay. In mice lethality assay, the mortality ratio was higher in 10 mg/kg piroxicam treated group. Conclusion: It is concluded from the present study that piroxicam may have immunosuppressant effects addition to its analgesic action and it may be clinically effective in autoimmune diseases and organ transplantation.
Piroxicam; Cell-mediated immunity; Humoral immunity
Zahoor B, Javeed A, ur Rehman N, Ahmed Tokhi, Zainab, Esmaeilpour K, et al. Evaluation of Cellular and Humoral Immune Responses of Piroxicam in Murine Model. Ann Pharmacol Pharm. 2020;5(3):1184.