Department of Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, ChinaFulltext PDF
Objective: To analyze the main capsid gene sequence and phylogeny of human Norovirus GII. Methods: The complete VP1 gene sequences of all strains GNoV GII.3 were aligned with Muscle of MEGA software. The VP1 gene distance was calculated by MEGA-X software to construct a phylogenetic tree, and the Bayesian Markov chain Monte Carlo method was used for phylogenetic analysis. Results: Nearly 29.1% of VP1 codon was strongly purified. More than 95% of the GII.3 norovirus cluster 2 strains were from China, and cluster 2 emerged about 4 years later than cluster 1. The similarity of VP1 gene shell domain was higher than that of convex domain, and the diversity of convex domain was the largest, reaching about 80%. Conclusion: The origin of GII.3 is later than that of GII.4. Under human immune pressure, HuNov was actively selected in vivo and became more adaptable to virus replication in vivo than ever before, with the potential to be an advantage in the future.
Human Norovirus GII.3; Phylogenetic tree; VP1 gene
Fang L. Phylogenetic Analysis of Human Norovirus GII.3 Based on Its Major Capsid Gene from 1972 to 2021. Am J Clin Microbiol Antimicrob. 2022; 5(1): 1060..