Am J Clin Microbiol Antimicrob | Volume 1, Issue 3 | Research Article | Open Access
Luiza Pinheiro1,2*, Isabella Carolina Rodrigues Dos Santos Goes3, Kesia Priscila Isquerdo Dias3, Lana Aparecida De Oliveira Pereira3, Caroline Rodrigues Costa3 and Valéria Cataneli Pereira3
1Department of Microbiology and Immunology, Institute of Biosciences, UNESP - Univ. State of São Paulo, Brazil
2Department of Pathology, Lauro de Souza Lima Institute, Brazil
3UNOESTE-Universidade do Oeste Paulista, Brazil
*Correspondance to: Luiza Pinheiro
Fulltext PDFElderly residents of nursing homes are more susceptible to bacterial infections when compared to young adults, mostly by commensal bacteria from microbiota, such as staphylococci. This study aimed to isolate S. aureus and S. epidermidis from the nasal cavity of elderly residents of a nursing home in Presidente Prudente, SP, Brazil, to determine their antimicrobial susceptibility and biofilm production. Swabs were collected from 32 individuals and the S. aureus and S. epidermidis isolates were tested regarding their antimicrobial susceptibility by the disc diffusion method and agar screening with oxacillin. Biofilm production was determined by the Congo Red Agar (CRA) method and adherence to borosilicate test tubes. Forty-one bacterial samples were obtained, corresponding to 87.8% of staphylococci. S. aureus was isolated from 10 individuals and S. epidermidis from 8. The isolates were resistant to oxacillin, cefoxitin, erythromycin, levofloxacin and clindamycin, and 100% susceptible to vancomycin and linezolid. Oxacillin resistance was found in 40% of S. aureus and 62.5% of S. epidermidis isolates. Biofilm production by S. aureus was observed in 90% of strains by CRA and 80% by the borosilicate test tube. By CRA and borosilicate tube test, respectively, 100% and 62.5% of S. epidermidis produced biofilm. Our data emphasize the need for prophylactic and biosecurity measures in order to avoid dissemination of multiresistant and virulent strains in those environments, a potential cause of elderly infections.
Pinheiro L, Dos Santos Goes ICR, Dias KPI, De Oliveira Pereira LA, Costa CR, Pereira VC. Antimicrobial Susceptibility and Biofilm Production by Staphylococcus aureus and Staphylococcus epidermidis Isolated from Elderly Residents of a Nursing Home. Am J Clin Microbiol Antimicrob. 2018; 1(3): 1011.