J Res Notes | Volume 5, Issue 1 | Research Article | Open Access

Determination of Multi-Drug Resistant and Extended Beta- Lactamase Producing Bacteria at Bethezatha Advanced Laboratory, Addis Ababa, Ethiopia

Zeleke W Tenssay

Bethezatha Hospital, Addis Ababa, Ethiopia

*Correspondance to: Zeleke W Tenssay 

Fulltext PDF


Background: Antimicrobial resistant bacteria are global health problems. Bacteria have several mechanisms to resist drug effects. Extended Spectrum Beta Lactamase (ESBLs) represents an impressive example of the ability of gram-negative bacteria to develop new antibiotic-resistance mechanisms in the face of the introduction of new antimicrobial agents. Assessing ESBL producing Enterobacteriaceae in the local scenario is necessary to understand the epidemiology and the disease burden as well as to design and implement hospital infection control strategies to prevent the further occurrence and spread of such bacteria. Materials and Method: A retrospective study of different clinical specimens (urine, blood culture, pus and discharges from different body sites) received by Bethzatha Advanced Laboratory for bacteriological culture and antimicrobial susceptibility test from different wards of Bethzatha Hospital and other Health Institutions in Addis Ababa. These were cultured for isolation and identification of bacterial pathogens using standard bacteriological culture media. MicroScan panels (Beckman Coulter, Brea, CA, USA) method was used for antibiotic susceptibility test and for extended spectrum bata-lactamase (ESBL) detection. Results: A total of 2112 clinical specimens were cultured at Bethzatha advanced Laboratory from May 2021 to July 2023. Most frequent isolate, 126 (58.6%) was E. coli. Of the total 216 gramnegative bacilli, 109 (51%) was found to be ESBL producers. Out of these, E. coli 68 (53.9%) were ESBLs producer followed by 21/41 (51%) Kllebsiella pneuomoniae, 9/19 (47.4%) P. mirabilis and 5/10 (50%) Enterobacter cloacae. Among ESBLs producing E. coli, 15/68 (22%) was resistant to six different antimicrobial drugs and one strain was resistant to 12 or more antimicrobials tested. Similarly, 4/21 (19%) and 2/5 (40%) K. pneumoniae and K. oxytoca were respectively resistant to six or more antimicrobial agents. Conclusion: In the present study multiply resistant and ESBL producing Gram negative bacilli were frequently isolated from different clinical specimens. Therefore, there must be periodic surveillance of antimicrobial resistance in hospitals and other health settings. Appropriate prescription of antimicrobial agents and avoiding misuse of antimicrobials is also important to minimize the spread of ESBL producing multidrug resistant bacterial strains.


MicroScan Pannel, Enterobacteriaceae; Extended Spectrum Beta Lactamase (ESBL); Antimicrobial Resistance


Tenssay ZW, Gebre A. Determination of Multi-Drug Resistant and Extended Beta-Lactamase Producing Bacteria at Bethezatha Advanced Laboratory, Addis Ababa, Ethiopia. J Res Notes. 2023; 5(1): 1025..

Subscribe to Our Newsletter