J Pathol Res Pract | Volume 1, Issue 1 | Research Article | Open Access
Mennat-Allah A Rehab*, Ayman M Elsaka, Hassan Tahseen Sheir and Hend S Abo Safia
Department of Pathology, Faculty of Medicine, Tanta University, Egypt
*Correspondance to: Mennat-Allah A Rehab
Fulltext PDFBackground: Hepatocellular Carcinoma (HCC) is one of the leading causes of cancer related deaths worldwide. Astrocyte Elevated Gene-1 (AEG-1) has a vital role in promoting cancer development and progression by augmenting all hallmarks of aggressive cancer. Glypican-3 (GPC3) is a heparansulfate cell surface proteoglycan expressed normally by fetal liver and is highly expressed in HCC. This study aimed to evaluate the diagnostic value of AEG-1 and Glypican-3 in HCC and precancerous lesions. In addition, the expression of the two markers with different grades of HCC was also evaluated. Material and Methods: Sixty cases; 36 cases of HCC and 24 cases of precancerous lesions, were subjected to routine hematoxylin and eosin staining and immunohistochemical staining using AEG-1 and GPC3. Results: AEG-1 was expressed in 94.4% of HCC cases and 12.5% of precancerous lesions with 94.4% sensitivity, and 87.5% specificity. GPC3 was expressed in 75% of HCC cases and 8.3% of precancerous lesions with 75% sensitivity, and 91.6% specificity. GPC3 expression has a statistically significant relation with high tumor grade. In contrast to AEG-1 that showed no statistically significant relation with the tumor grade. The combination of both markers provided better sensitivity (98.2%), and absolute specificity (100%). Conclusion: AEG-1 showed higher sensitivity and diagnostic accuracy than GPC3, while GPC3 showed more specificity. Combined AEG-1 and GPC3 immunohistochemical panel can be used efficiently for accurate diagnosis of HCC and differentiating HCC from precancerous lesions.
HCC; Precancerous lesions; AEG-1; GPC3
Mennat-Allah A Rehab, Elsaka AM, Sheir HT, Abo Safia HS. The Diagnostic Utility of Both Astrocyte Elevated Gene-1 (AEG-1) and Glypican-3 Immunohistochemical Expression in Hepatocellular Carcinoma. J Pathol Res Pract. 2024; 1(1): 1001..