J Hematol Mult Myeloma | Volume 2, Issue 1 | Privileged Communication | Open Access

The Histone H2A Deubiquitinase Usp16 is Required for Mouse Fetal Hematopoiesis

Yue Gu and Hengbin Wang^*

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, USA

^*Correspondance to: Yue Gu and Hengbin Wang 

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Abstract

Hematopoiesis, the process by which all blood cells are generated, is controlled by an interactive network of transcription factors and epigenetic regulators [1-3]. In previous studies, we discovered that USP16, a histone H2A-specific deubiquitinase, plays an indispensable role in mouse adult hematopoiesis and is require for hematopoietic stem cell (HSC) to undergo differentiation [4]. In this study, we investigated the function of Usp16 in mouse fetal hematopoiesis by crossing Usp16 conditional knockout mice with VavCre mice. While Usp16KO E14.5 embryos appear normal, E17.5 Usp16KO embryos are pale. The frequencies of LSK cells are increased and the frequencies of MyePro cells are decreased in these embryos. While the absolute number of long-term HSC is not changed, there is a significant decrease of ST-HSC numbers. This study reveals that Usp16 is required for mouse fetal hematopoiesis and Usp16 regulates long-term HSC to ST-HSC transition during fetal hematopoiesis.

Citation:

Gu Y, Wang H. The Histone H2A Deubiquitinase Usp16 is Required for Mouse Fetal Hematopoiesis. J Hematol Mult Myeloma. 2017;2(1):1008.