J Gynecol Oncol | Volume 1, Issue 1 | Research Article | Open Access
Tao Cui1,2, Shang-Wei Li1,2, Shan Luo1,2, Lang Qin1,2, Xun Zeng1,2 and Xiao-Hong Li1,2*
1Department of Obstetrics and Gynecology, Sichuan University, China
2Department of Birth Defects and Related Diseases of Women and Children, Sichuan University, China
*Correspondance to: Xiao-Hong Li
Fulltext PDFBackground: To explore the expression of HSP70 in chronic unpredictable mild stress (CUMS) mouse embryos cultivated in vitro.
Methods: We obtained 452 two-cell embryos from 39 CUMS mice and 137 ones from 7control mice, and cultivated them in vitro in M2 medium at 37o C and 5% CO2 . Forty-eight hours later, blastocysts were collected and HSP70 expression was analyzed with Immuno Fluorescence (IF) staining and Real-Time Polymerase Chain Reaction (RT-PCR). Twenty-four hours later, morulae were collected for transfecting HSP70-si RNA by electroporation to down-regulation HSP70 and blastocystim plantation rate was assayed.
Results: The blastocyst formation rate in experimental group (38.2%) was significantly lower than in the control group (52.6%, P< 0.05). Compared with the control group (5.01±2.11), the level of HSP70 mRNA in experimental group blastocysts (3.35±1.23) also decreased significantly (P< 0.05). After transfectingHSP70-siRNA to a morula of experimental group by electroporation, the level of HSP70 mRNA in the HSP70-siRNA group (1.01±0.58) was significantly lower than that in the no electroporation group (3.71±1.29) (P < 0.05). And the embryo implantation rate in experimental group (18.8%) was significantly lower than in control group (50%, P< 0.05). Conclusions: Inducible HSP70 may play a protective role in the CUMS embryos in vitro.
Heat shock protein 70; Chronic Unpredictable Mild Stress; in vitro; siRNA; Electroporation
Cui T, Li S-W, Luo S, Qin L, Zeng X, Li X-H. Inducible HSP70 May Play a Protective Role in CUMS Mouse Embryos In Vitro. J Gynecol Oncol. 2018; 1(1): 1003.