J Clin Obstet Gynecol Infertil | Volume 7, Issue 2 | Research Article | Open Access
Cuili N1, Juan L1, Dongfang L1, Xiaolin C1, Yanmin M1, Chen X1 and Ye Z2*
1Department of Gynecology, The Third Hospital of Xingtai, China 2Department of Gynecology, The Second Affiliated Hospital of Xingtai Medical College, China
Fulltext PDFObjective: To investigate Mitogen Activated Protein Kinase 4 (MAP4K4) and Hypoxia-inducible in peripheral blood of patients with locally advanced cervical cancer the relationship between the expression of factors 2α, HIF-2α and autophagy overactivation and sensitivity to platinum-type neoadjuvant chemotherapy. Methods: 230 patients with locally advanced cervical cancer admitted to our hospital from August 2017 to September 2020 were prospectively selected as research objects. The expression of MAP4K4, HIF2α and autophagy related genes (LC-3 II, LC-3 I and Beclin-1) in peripheral blood before and after chemotherapy were detected by qRT-PCR. Pearson test was used for correlation analysis. The independent risk factors of chemotherapy sensitivity was analyzed by logistics regression model. Results: The expression of MAP4K4 and HIF2α in peripheral blood of patients with locally advanced cervical cancer was observed among subgroups of age, tumor size and histological type (P>0.05), but was observed among subgroups of differentiation, FIGO stage and lymph node metastasis (P<0.05). Before chemotherapy, MAP4K4 mRNA and HIF2α mRNA in peripheral blood of the two groups were compared (P>0.05). After chemotherapy, MAP4K4 mRNA and HIF2α mRNA in both groups were significantly decreased (P<0.05), and MAP4K4 mRNA and HIF2α mRNA in the effective group were significantly lower than those in the ineffective group (P<0.05). Before chemotherapy, LC-3 II, LC-3 I and Beclin-1 were compared between the two groups (P>0.05). After chemotherapy, LC-3 II, LC-3 I and Beclin-1 in both groups were increased, and LC-3 II, LC-3 I and Beclin-1 in ineffective group were significantly higher than those in effective group (P<0.05). MAP4K4 and HIF2α were significantly positively correlated with LC-3 II, LC-3 I and Beclin-1 (P<0.05). Differentiation degree, FIGO stage, lymph node metastasis, LC-3 II, LC-3 I, Beclin-1, MAP4K4 and HIF2α were independent risk factors for chemotherapy sensitivity in patients with locally advanced cervical cancer (P<0.05). Conclusion: The up-regulated expression of MAP4K4 and HIF2α in patients with locally advanced cervical cancer is closely related to over-activation of autophagy during chemotherapy and sensitivity to platinum neoadjuvant chemotherapy.
Locally Advanced Cervical Cancer; MAP4K4; HIF2α; Hyperactivation of Autophagy; Sensitivity to Platinum Neoadjuvant Chemotherapy
Cuili N, Juan L, Dongfang L, Xiaolin C, Yanmin M, Chen X, et al. Relationship Between the Expression of MAP4K4 and HIF2α in Peripheral Blood of Patients with Locally Advanced Cervical Cancer and Over-Activation of Autophagy and Sensitivity to PlatinumType Neoadjuvant Chemotherapy. J Clin Obstet Gynecol Infertil. 2023; 7(2): 1061.