J Clin Obstet Gynecol Infertil | Volume 4, Issue 1 | Research Article | Open Access
Elsharkawy SS1*, Abdrabo MS1, khalil MH1 and Mannaa HF2
1Department of Obstetrics and Gynecology, Alexandria University, Egypt 2Department of Medical Biochemistry, Alexandria University, Egypt
*Correspondance to: Elsharkawy SS
Fulltext PDFObjective: To study the levels of four markers of oxidative stress in Follicular Fluid (FF) and serum of patients with infertility related to endometriosis and controls. Design: Experimental study. Setting: University hospital and infertility center. Patient(s): Sixty infertile women were included in the study (30 infertile women with endometriosis and 30 controls including infertile women due to male factor, or unexplained infertility). Intervention(s): Blood was obtained at the time of egg retrieval, and FF from the mature follicles of each ovary was centrifuged and frozen until analysis. Main outcome measure(s): Vitamin C and E, malondialdehyde, and superoxide dismutase concentrations in serum and follicular fluid. Result(s): Women with endometriosis showed a lower vitamin C concentration in serum (1.64 μg/ ml to 11.48 μg/ml vs. 6.56 μg/ml to 41.33 μg/ml) and higher superoxide dismutase concentration in FF (30.41 U/mL to 339.53 U/mL vs. 70.95 U/mL to 324.32 U/mL) compared with controls. Vitamin E FF levels were significantly lowers in women with endometriosis (0.0 μg/mL to 0.52 μg/ mL vs. 0.04 μg/mL to 1.38 μg/mL). A non significant difference in serum and FF concentration of malondialdehyde was found in women in both groups. Conclusion(s): These findings suggest increased oxidative stress in infertile women with endometriosis, which may be manifested by increased activity of SOD in FF and/or consumption of the antioxidant vitamin C in serum, and decrease level of vitamin E in FF of the same affected group.
Oxidative stress; Endometriosis; Infertility
Elsharkawy SS, Abdrabo MS, khalil MH, Mannaa HF. Evaluation of Oxidative Stress Markers in Serum and Follicular Fluid of Women with Infertility Related to Endometriosis. J Clin Obstet Gynecol Infertil. 2020; 4(1): 1045.