Jpn J Cancer Oncol Res | Volume 2, Issue 1 | Research Article | Open Access

Regulation of DNA Methylation in Female Subjects with Increased (Pathological) Level of Circulating Immune Complexes and Breast Tumours

Michael Chukwudi Ezeani1*, Charles Chinedum Onyenekwe2, Samual Chukwuemeka Meludu3, Ujuamala Uloma Ezeani4, Gabriel Chianakwana5, Martin Ositadimma Ifeanyichukwu2 and Comfort Nne Akujobi6

1Department of Immunology, Nnamdi Azikiwe University, Nigeria
2Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nigeria
3Department of Human Biochemistry, Nnamdi Azikiwe University, Nigeria
4Department of Medical Centre, Nnamdi Azikiwe University, Nigeria
5Department of Surgery, Nnamdi Azikiwe University, Nigeria
6Department of Medical Microbiology/Parasitology, Nnamdi Azikiwe University, Nigeria

*Correspondance to: Michael Chukwudi Ezeani 

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Abstract

Background: Pathological effects of circulating immune complexes have not been extensively explored, in the fields of oncology, considering its potentials to mediate immuno-pathological effects. The purpose of this study was to assess DNA methylation patterns in breast tumours and apparently healthy female subjects with increased levels of circulating immune complexes.
Methods: We assayed serum samples from 50 apparently healthy female volunteers, 49 subjects with benign and malignant breast tumours (24 with benign tumour and 25 with malignant tumour) for circulating immune complexes using Polyethylene Glycol (PEG) immunoprecipitation, Isolated cell free DNA (FitAmp blood kit (Epigentek, USA) was assayed for global decrease or increase in 5-methylcytosine (5-mC) using immunoassay (Colorimetric) method.
Results: The proportion of subjects with benign and malignant tumours demonstrating increased level of CIC was 70.8% (17 of 24 subjects) and 96% (24 of 25 subjects) respectively and 32% (16 of 50 subjects) in apparently healthy subjects. Between DNA methylation aberration markers, the frequency of hypomethylation and unmethylation were significantly high in benign, malignant and healthy subjects with increased level of circulating immune complexes. Expression of normal methylation was significantly high in healthy control subjects with normal level of circulating immune complexes. Strong positive correlation was obtained between levels of immune complexes and DNA methylation shifts P=0.000.
Conclusion: DNA methylation aberration (hypomethylation and unmethylation) in the global CpG regions were synonymous with increased level of circulating immune complexes. This revealed possible pathological effect of immune complexes on DNA methylation patterns in healthy and in diseased conditions.

Citation:

Ezeani MC, Onyenekwe CC, Meludu SC, Ezeani UU, Chianakwana G, Ifeanyichukwu MO, et al. Regulation of DNA Methylation in Female Subjects with Increased (Pathological) Level of Circulating Immune Complexes and Breast Tumours. Jpn J Cancer Oncol Res. 2019; 2(1): 1006.

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