Ann Surg Case Rep | Volume 7, Issue 1 | Research Article | Open Access
Qianman, Jiasharete, Yeerjiang, Wupuer, Tiangkejie, Halpat, Buranbai, Jialihasi, Shawutali, Aihemaiti, Maimaiti, Alimujiang, Tieliuhan, Ydyrys, Aisaiding, Yeerbo, and Jielile*
Department of Orthopedics & Ankle Surgery, The Sixth Teaching Hospital of Xinjiang Medical University, China
School of Basic Medical Sciences, Capital Medical University, Beijing, China
Department of Medical Records Management, The Sixth Affiliated Teaching Hospital of Xinjiang Medical University, China
Qitai Hospital of the 6th Division, Xinjiang Production and Construction Corps, Qitai County, China
Division of Child Neurology, Tottori University, Japan
Department of Orthopedics, Almaty Medical Center HAK, Kazakhstan
Department of Pediatric Surgery, The People's Hospital of Xinjiang Uygur Autonomous Region, China
Department of Orthopedics Centre, The First Teaching Hospital of Xinjiang Medical University, China
Department of Central Institutes of Health, Kupu Township, China
Al-Farabi Kazakh National University, Biomedical Research Center, Kazakhstan
Department of Hand and Foot Microsurgery, Children's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Department of Geriatric Joint Surgery of Orthopedics, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
The authors contributed equally to this study.
*Correspondance to: Jiasharete Jielile
Fulltext PDFBackground: The traditional medicine using Horse Oil Percutaneous Permeation (HOPP) has been recommended for the treatment of ankylosis. In this study, using a quantitative proteomics approach, we investigated the therapeutic mechanism of HOPP in a rat ankylosis model. Methods: Sixty Sprague-Dawley (SD) rats with ankle joint ankylosis were randomized to a treatment group that received HOPP (n=30) or a control group (n=30). Synovial tissue samples from the ankle joint were collected at 7, 14, and 21 days in each group. Protein levels were assessed using isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Differentially expressed proteins subjected to bioinformatics analysis. Results: Among 1,729 proteins identified in synovial tissue samples, 200 proteins were differentially expressed across three time points between the HOPP treatment and control groups. Further analysis revealed that the differentially expressed proteins are involved in energy metabolism, calcium metabolism, myosin proteins, and mitogen-activated protein kinase signaling, and thus, these may represent the major mechanisms of the therapeutic effects of HOPP. Conclusion: The results provide mechanistic insight into the improved joint function achieved with HOPP treatment, and thus, could be useful in the development of improved clinical treatments for anchylosis.
Ankylosis; Arthrofibrosis; Traditional medicine; horse oil; iTRAQ; Proteomics; Therapeutic mechanism.
Qianman B, Jiasharete T, Yeerjiang Y, Wupuer A,Tiangkejie W, Halpat A, et al. Quantitative Proteomic Analysis Provides Insight into the Therapeutic Mechanism of Horse Oil Percutaneous Permeation Treatment in a Rat Ankylosis Model. Ann Surg Case Rep. 2024; 7(2): 1090. .