Ann Stem Cells Regen Med | Volume 1, Issue 1 | Short Communication | Open Access
María Alejandra Pardo-Pérez1, Claudia Camila Mejía-Cruz1, Efraim Leal-García2, Rafael Pérez-Núñez2, Luis Fernando Useche-Gómez2, Viviana Marcela Rodríguez-Pardo1*
1Immunobiology and Cell Biology group. Department of Microbiology. Javeriana University, Colombia
2Department of Orthopedics and Traumatology Faculty of Medicine, Pontificia Universidad Javeriana, Colombia
*Correspondance to: Viviana Marcela RodríguezPardo
Fulltext PDFBackground: Mesenchymal Stromal Cells (MSC) have a great importance for the field of regenerative medicine. However, there is high variability in existing protocols for MSC in vitro expansion, which can lead to low reproducibility of pre-clinical studies and, even more critically, the reduced safety of patients undergoing clinical trials. Although bone marrow is one of the most important sources for the isolation and in vitro culture of MSC, the preferred anatomical location for obtaining bone marrow is often unclear, and this information is relevant for the interpretation of results obtained from preclinical and clinical trials.
Methods: In this study, we compared various biological characteristics of human MSC obtained from five total hip replacement surgery donors isolated from the bone marrow of two different anatomical sites: the femoral head bone (Fh) and the acetabular subchondral bone (Ac). Using the same surgical technique and collection volume, we compared the morphological characteristics, fibroblast colony forming unit (CFU-F) capacity, immunophenotype, capacity for differentiation (osteogenesis, condrogenesis, and adipogenesis) and population doubling time (PDT) of MSC isolated from these distinct anatomical locations.
Results: Here, we show that human MSC isolated from Fh have improved morphological and proliferative characteristics that are associated with higher in vitro efficiency for regenerative medicine protocols compared with cells obtained from Ac.
Conclusion: This report provides information regarding the importance of establishing a "standard" anatomical site for obtaining bone marrow as one of the most important requirements of the "preanalytical" phase of MSC in vitro expansion for regenerative medicine
Pardo-Pérez MA, Mejía-Cruz CC, LealGarcía E, Pérez-Núñez R, UsecheGómez LF, Rodríguez-Pardo VM. Femoral Head Bone vs Acetabular Subchondral Bone: Selecting the Optimal Anatomical Site to Obtain Mesenchymal Stromal Cells from Human Bone Marrow for Regenerative Medicine. Annals Stem Cell Regenerat Med. 2018;1(1):1005.