Ann Short Rep | Volume 2, Issue 1 | Research Article | Open Access
Li Xing, Houyu Ma and Yelian Miao*
Department of College of Food Science and Light Industrial Technology, Nanjing Tech University, China
*Correspondance to: Yelian Miao
Fulltext PDFCerebrosides A and B (A-B) are sphingolipids with high neuro protective activities. The present study aimed to validate the role of serine as a precursor for the biosynthesis of cerebrosides A-B by Termitomyces clypeatus CTM-1. Mycelium culture of T. clypeatus CTM-1 was carried out on a rotary shaker, using media with different serine doses in the range of 0 to 1.0 g/L. At the serine dose of 0.75 g/L, the cerebroside a content of mycelium reached a maximum of (0.30 ± 0.02)%, while the cerebroside B content increased to (0.10 ± 0.01)% in 9 days. The cerebroside levels were about 36% and 11% higher than those at a serine dose of 0, respectively. It was clear that a moderate level of serine in mycelium-culture medium might be used as a precursor to enhance significantly the biosynthesis of cerebrosides A-B.
Bioactive compounds; Cerebroside; Termitomyces clypeatus; Mycelium; Biosynthesis; Precursor
Xing L, Ma H, Miao Y. Biosynthesis of Cerebroside by Termitomyces clypeatusUsing Serine as a Precursor. Ann Short Reports. 2019;2:1030.