Ann Pediatr Res | Volume 4, Issue 5 | Research Article | Open Access

The Effect of High Dose Methotrexate on Thiol/Disulfide Homeostasis in Pediatric Cancer Patients

Sonay Incesoy Ozdemir^1*, Gurses Sahin², Emre Capkınoglu², Ali Fettah², Sule Yesil², Burcak Bilgin², Nilgun Eroglu² and Ozcan Erel³

¹Department of Pediatric Hematology Oncology, Ankara Yıldırım Beyazıt University, Turkey ²Department of Pediatric Hematology Oncology, Dr. Sami Ulus Maternity and Children's Health and Diseases Training and Research Hospital, Turkey ³Department of Biochemistry, Ankara Yıldırım Beyazıt University, Turkey

^*Correspondance to: Sonay Incesoy Ozdemir 

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Abstract

Objectives: Current literature shows that there is a relationship between oxidative stress and Methotrexate (MTX). However, there are no clinical studies evaluating oxidant status in children treated with High Dose MTX (HDMTX) in the literature. Therefore, we aimed to evaluate the effects of HDMTX on thiol/disulfide balance in pediatric cancer patients. Material and Methods: The study sample consisted of 19 patients with leukemia and lymphoma who had received 5 g/m2 doses of MTX. All blood samples for hemogram, serum biochemistry, and thiol and disulphide analysis were collected at 0 and 48 h after starting the MTX infusion. Results: Native thiol was 320.21 𝜇mol/L (280.36 to 392.38) before treatment and 409.78 𝜇mol/L (335.13 to 451.67) after treatment. The median post-treatment levels were found to be significantly higher than the median pre-treatment levels of native thiol (409.78 𝜇mol/L and 320.21 𝜇mol/L, respectively) and total thiol (441.61 𝜇mol/L and 377.46 𝜇mol/L, respectively) (p<0.05). A positive correlation was found between leukocyte, neutrophil, albumin, total thiol and native thiol levels before and after treatment. Conclusion: This is the first study that highlights an association between thiol/disulphide homeostasis and HDMTX treatment. We think that these findings enhance our understanding about the relationship between oxidative stress and MTX.

Keywords:

Children; Dynamic thiol/disulphide homeostasis; High dose methotrexate; Pediatric cancer; Leukemia; Lymphoma

Citation:

Ozdemir SI, Sahin G, Capkınoglu E, Fettah A, Yesil S, Bilgin B, et al. The Effect of High Dose Methotrexate on Thiol/Disulfide Homeostasis in Pediatric Cancer Patients. Ann Pediatr Res. 2020;4(5):1050..