Govindan Ramachandran1, Govindan Rajivgandhi1*, Muthuchamy Maruthupandy2 and Natesan Manoharan1
1Department of Marine Science, Bharathidasan University, India
2Department of Packaging, Yonsei University, Korea
In present investigation, the antibacterial activity of Endophytic Actinomycetes (EA) isolated from mangrove plant of Avicennia marina in Muthupet Mangrove region, South East Coast of Tamil Nadu, India. The EA were isolated from Actnomycetes Isolation Agar (AIA) plate method after surface sterilization. After isolation, all the strains were detected their antibacterial activity against Multi Drug Resistant Uropathogens (MDRU) by conventional cross streak method. Further, the active strain was selected and crude extract was purified with various solvent by liquid-liquid extraction method. Then, the active solvent was chosen for further purification for antibacterial compound against selected MDRU including Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), Pseudomonas aeruginosa (P. aeroginosa), K. Pnumoniae(K. pneumonia) and Enterobactersp. In result, the 100 strains of EA were showed white, creamy, powdery growth of the colony after surface steriliztation. The validation of surface sterilized water proved that the isolates were emerged from internal tissue of the plant, refereed as endophytes. Among the 100 strains, 10 strains were exhibited better antogonistic activity against selected MDRU strains. Based on the antogonistic activity, the excellent antibacterial activity of NM 5 was chosen for further study. The antibacterial potential of the NM 5 crude extract was purified by ethyl acetate extract and showed excellent inhibition activity 22, 17, 18, 20, and 16 mm against E .coli, P. aeroginosa, K. pneumonia and Enterobactersp, were observed respectively. The Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the purified crude extract were showed excellent inhibitory activity at 100 μg/ml against selected uropathogens. This concentration was used for further studies like anti-fungal, anti-cancer, anti-biofilm and larvicidal activity.
Entophytic actinomycetes; Multi drug resistant bacteria; Minimum inhibition concentration; Gram negative bacteria; Antibacterial activity
Ramachandran G, Rajivgandhi G, Maruthupandy M, Manoharan N. Isolation and Identification of Antibacterial Compound from Marine Endophytic Actinomycetes against Multi Drug Resistant Bacteria. Ann Microbiol Immunol. 2018; 1(1): 1003.