Ann Med Med Res | Volume 6, Issue 1 | Research Article | Open Access
Kamranfar F1, Hashemzadeh S2, Arjmand A1, Mousavi Z2* and Pourahmad J1*
1Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical
Sciences, Iran
2Department of Toxicology and Pharmacology, Tehran Medical Sciences, Islamic Azad University, Iran
*Correspondance to: Jalal Pourahmad
Fulltext PDFOxidative stress and mitochondrial dysfunction are considered as the main nephrotoxicity
mechanisms in uranyl acetate-induced renal damage. The delivery of freshly isolated mitochondria
into a damaged cell from healthy tissue is one of the new treatments for mitochondria-related
diseases. Transfer of exogenous mitochondria may restore normal cell function.
The effectiveness of inserting newly isolated mitochondria from the rat kidney into uranyl acetatedisrupted
rat Renal Proximal Tubular Cells (RPTCs) was the focus of this investigation. By
measuring changes in Mitochondrial Membrane Potential (MMP) and mitochondrial Succinate
Dehydrogenase Activity (SDH), the proper functioning of isolated mitochondria was initially
characterized.
Afterward, the protective effects of mitochondrial transplantation on the impaired RPTCs were
assessed through several parameters including MTT, Reactive Oxygen Species (ROS), MMP, lipid
peroxidation, GSH and GSSG contents and caspase-3 activity. Our data showed that addition of
exogenous mitochondria to the uranyl acetate -treated RPTCs, significantly enhanced cell survival
by decreasing of ROS generation, MMP collapse and lipid peroxidation, increasing of GSH contents
and reducing of GSSG levels and caspase-3 activity.
In conclusion, our findings demonstrated that mitochondrial transplantation might be a promising
option for treating the nephrotoxic effects of uranyl acetate.
Uranyl acetate; Mitochondrial transplantation; Nephrotoxicity; Oxidative stress
Kamranfar F, Hashemzadeh S, Arjmand A, Mousavi Z, Pourahmad J. Mitochondrial Transplantation Improved Renal Damage - Induced by Uranyl Acetate. Ann Med Medical Res. 2023; 6: 1064..