Ann Med Med Res | Volume 6, Issue 1 | Research Article | Open Access

N-Acetylcysteine can Suppress Mitochondria-Dependent Apoptosis of Macrophages Caused by Burkholderia pseudomallei Infection

Chen C^1,2#, Zeng Y^1#, Li X^1#, Luo J¹, Chen Y¹, Xu X¹, Pei H^1*, Gu S^3* and Xia Q^1*

¹Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical
Disease Control, School of Tropical Medicine and The Second Affiliated Hospital, Hainan Medical University, China
²International School of Public Health and one Health, Hainan Medical University, Haikou, Hainan, China
³Department of Neurosurgery, Neurology Center, The First Affiliated Hospital of Hainan Medical University, China
#These authors contributed equally to this work

^*Correspondance to: Hua Pei 

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Abstract

Melioidosis, a zoonotic infection primarily caused by Burkholderia pseudomallei infection, suppresses the host immune response and induces apoptosis. Excessive Reactive Oxygen Species (ROS) can trigger mitochondrial-dependent apoptosis and oxidative stress may be an important mechanism in B. pseudomallei-mediated cytotoxicity. In this study, the changes of ROS in macrophage apoptosis induced by B. pseudomallei were investigated. Our results showed that B. peudomallei infection (MOI = 0, 1, 5, 10) for 24 h induces ROS overproduction, regulating protein expression involved in oxidative stress and leading to mitochondrial apoptosis. Furthermore, pretreatment with antioxidant N-Acetylcysteine (NAC) for 2 h significantly reduced oxidative damage and mitochondrial damage in mouse macrophages infected with B. pseudomallei, suggesting its potential for regulating mitochondria-dependent apoptosis in infected macrophages. In summary, our results emphasize the effects of ROS in B. pseudomallei pathogenesis, and NAC inhibits mitochondria-dependent apoptosis of macrophages induced by B. pseudomallei infection by reducing ROS production.

Keywords:

Keywords: Burkholderia pseudomallei; Apoptosis; Mitochondrial damage; Oxidative stress; Infection

Citation:

Chen C, Zeng Y, Li X, Luo J, Chen Y, Xu X, et al. N-Acetylcysteine can Suppress Mitochondria-Dependent Apoptosis of Macrophages Caused by Burkholderia pseudomallei Infection. Ann Med Medical Res. 2023; 6: 1062..