Ann Med Med Res | Volume 6, Issue 1 | Research Article | Open Access

Therapeutic Effects of Malva sylvestris Extract on Polycystic Ovary Syndrome in a Rat Model

Bayat M1, Rahimi-Feyli P1*, Azadbakht M2, Moghaddam A1 and Goodarzi N3

1Department of Clinical Sciences, Razi University, Kermanshah, Iran
2Department of Biology, Razi University, Kermanshah, Iran
3Department of Basic Sciences, Razi University, Kermanshah, Iran

*Correspondance to: Peyman Rahimi-Feyli 

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Objective: Polycystic Ovary Syndrome (PCOS) is known as one of the most usual hormone
disorders in women. Malva sylvestris is a plant with a potent antioxidant effect. Accordingly, this
research aimed to investigate the effect of this herb on ovarian folliculogenesis using a rat model of
testosterone enanthate induced PCOS.
Methods: In this experiment, 35 immature female Wistar rats were used. The rats in the control
group (Control) only received sesame oil as testosterone enanthate solvent, whereas the rats in the
PCOS (PCOS control group) were given daily subcutaneous injections of testosterone enanthate (1
mg/100 g body weight dissolved in sesame oil) for 28 days. Rats in the extract groups were given
50 (P+E50), 100 (P+E100), and 200 (P+E200) mg/Kg BW hydroalcoholic extract of Malva sylvestris by
gavage for 4 weeks. At the end of the experiment, the animals were anesthetized, then blood samples
were collected and the ovary was subjected to histological analyses. The data were analyzed using the
one-way ANOVA method considering P<0.05/level of significance.
Results: Rats treated with testosterone enanthate (PCOS control group) demonstrated a significant
increase in serum testosterone, and LH, and a significant decline in progesterone. Also, disturbing
ovarian cyclicity in addition to histopathological alterations, including decreased number of
healthy follicles and corpora lutea, increased degenerated, and cystic follicles were detected by light
microscopic studies. Rats treated with an extract of Malva sylvestris (P+E50; P+E100; P+E200) showed a
remarkable reversal in the levels of parameters affected by testosterone enanthate treatment.
Conclusion: In conclusion, Malva sylvestris extract may be an effective therapeutic candidate for the
treatment of PCOS. It seems that the hydroalcoholic extract of Melia azedarach L. seed may lead to
a normal ovarian cycle by reducing the androgen concentration.


Ovary; PCOS; Wistar rats; Testosterone enanthate; Malva sylvestris


Bayat M, Rahimi-Feyli P, Azadbakht M, Moghaddam A, Goodarzi N. Therapeutic Effects of Malva sylvestris Extract on Polycystic Ovary Syndrome in a Rat Model. Ann Med Medical Res. 2023; 6: 1055..

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