Ann Med Med Res | Volume 7, Issue 1 | Research Article | Open Access
Corpuz IMB1,2, Arbasto Jr. FT1,2, De Guzman AAS1,2, Devilles CJU1,2, Drepite LNC1,2, Eugenio WJE1,2, Goboy AF1,2, Guhil MEL1,2, Iligan JMP1,2*, Lagasca GMM1,2, Orias CTG1,2, Ramoso GG1,2, Ringor LCL1,2, Rubio SMWB1,2, Sabaybay RM1,2, Solasco MAR1,2, Susaya GAB1,2, Tayag ACN1,2, Trinidad RKC1,2, Villareal AD1,2, Hermogenes EGR1,2,3 and Almonidovar MR1,2,3
1College of Medical Laboratory Science, Philippines 2Our Lady of Fatima University, Philippines 3Research Development and Innovation Center, Philippines
*Correspondance to: Jahn Mae P Iligan
Fulltext PDFCancer remains a significant health threat to humanity, as it continues to develop multidrug resistance to treatments and makes anti-cancer drugs ineffective. The study sought to use methanolic extract of Leea manillensis (locally known as Abang-Abang) against MCF-7 breast adenocarcinoma cell line. The antioxidant activity of L. manillensis was measured by DPPH Free radical scavenging assay, while the cytotoxicity was observed using PrestoBlue Assay on MCF-7 cells. The Inhibitory Concentration (IC50) values of L. manillensis and Doxorubicin were 2724 μg/mL and 0.7644 μg/mL respectively. Among the five concentrations of L. manillensis extract, the 60 μg/mL concentration displayed a high effectivity rate of 38.99% in DPPH Assay, while the ascorbic acid had 99.52%. Results showed that both positive controls from the tests performed obtained higher effectivity than the plant extract. The methanolic extract of Leea manillensis is ineffective in DPPH scavenging assay as well as in inhibiting viability of MCF-7 cells using the PrestoBlue Assay.
Leea manillensis; MCF-7 Breast Adenocarcinoma Cell line; Cytotoxicity; Antioxidant
Corpuz IMB, Arbasto Jr. FT, De Guzman AAS, Devilles CJU, Drepite LNC, Eugenio WJE. In Vitro Cytotoxicity and Antioxidant Study of Leea manillensis (Abang-Abang) Leaf Methanolic Extract against MCF-7 Breast Adenocarcinoma Cell. Ann Med Medical Res. 2024; 7: 1080..