Department of Chemistry, Siedlce University of Natural Sciences and Humanities, PolandFulltext PDF
The aim of this study was to investigate the ability of synthesized pyrazolo[4,3-e][1,2,4]triazine sulfonamide compounds to affect development of Cytotoxic T Lymphocytes (CTL) in vitro in mixed leukocyte cultures. Compounds were dissolved in DMSO (1 mM) and added to cultures of mouse lymphocytes (BL/6) stimulated with irradiated allogeneic cells (BALB/c). Following 6 days of culture, cells were harvested from all cultures, washed, and assayed in 5 hr 51Cr release assays with labeled P815 tumor target cells for analysis of CTL with BL/6 anti-BALB/c specificity. All assays were performed on three separate occasions, and data analyzed for evidence of compounds able to produce enhancement or suppression of CTL activity, compared with control cultures receiving DMSO only. As show below, several compounds with reproducible enhancement/suppressive activity were documented.
Sulfonamides; Pyrazolo[4,3-e][1,2,4]triazine; Biological activity; Cytotoxic T Lymphocytes (CTL)
Mojzych M. Biological Activity Evaluation of Pyrazolo[4,3-e][1,2,4] Triazine Sulfonamides. Ann Med Chem. 2019;1(1):1001.