Ann Infect Dis Epidemiol | Volume 1, Issue 1 | Research Article | Open Access
Page Robert W, Thompson Iain JT, Rowland Caroline A, Clark Graeme C and Williamson E Diane*
Defence Science and Technology Laboratory, UK
*Correspondance to: Williamson E Diane
Fulltext PDFDendritic cells pulsed with Burkholderia pseudomallei were used to activate naive T-cells in vitro. Murine bone-marrow- derived dendritic cells (BMDC), cultured with CpG and GM-CSF or CpG and Flt3L ex vivo, were pulsed with killed B. pseudomallei or the Burkholderia protein Lolc, and cocultured with naïve T-cells. GM-CSF- derived BMDC pulsed with B. pseudomallei and CpG were activated, with CD40, CD80, CD86 and MHCII expression. Lolc, in the absence of CpG, was not immunostimulatory. GM-CSF-cultured BMDC, pulsed with B. pseudomallei and CpG, expressed CCR7 and imprinted CCR9 on co-cultured naïve T-cells, unlike BMDC cultured with FLt3L and CpG. GM-CSF-cultured, but not Flt3L-cultured BMDC pulsed with B. pseudomallei and CpG, had significantly up-regulated ALDH activity in the retinoic acid pathway. CCR9 expression on T-cells activated by GM-CSF-cultured BMDC pulsed with B. pseudomallei and CpG, was undiminished by pre-exposing BMDC to lung, spleen or skin tissue factors. Previously, we have observed that GM-CSF and CpG- cultured B. pseudomallei-pulsed BMDC, induced protective efficacy against B. pseudomallei challenge on adoptive transfer into naïve mice. The current data suggest that the protective mechanisms induced in vivo, are associated with the influence of GM-CSF and CpG on DC, to up regulate ALDH and to imprint B. pseudomalleispecificity and migratory potential on co-cultured T cells.
CCR9; T-cells; BMDC; GM-CSF; FLT3L; CpG
Page Robert W, Thompson Iain JT, Rowland Caroline A, Clark Graeme C, Williamson E Diane. Differentiation of BMDC with CpG and Gm-CSF, But Not CpG and Flt3L, Imprints CCR9 Expression on Co-Cultured T Cells. Ann Infect Dis Epidemiol. 2016; 1(1): 1001.