Ying-Chung Lee, Tien-Wang Chuang, Wen-Jye Lin and Chien-Wei Hou*
Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, TaiwanFulltext PDF
The tuyama is a precious Chinese medicine contains gastrodin and other constituents and commonly used for the treatment of neurodegenerative disorders. The protective effects of the tuyama and gastrodin, against Kainic Acid (KA) induced seizure have not been studied clearly. We investigated the effect of the tuyama extract and gastrodin on KA-induced neuronal injury in vitro and the tuyama extract-assisted sedative function by human HRV. The results confirmed that they provided effective protection against the excitatory damage of PC12 and BV-2 neuronal cells subjected to kainate. They could remove the ROS/NO, reduce MDA, PGE2 production, and caspase-3 protein. Western results revealed that p-ERK MAPK and COX-2 expression were significantly reduced by the tuyama and gastrodin. Summary, this study demonstrated that the tuyama and gastrodin provided a neuroprotective effect against excitotoxin and in part of involves with inhibiting p-ERK MAPK cell signal.
Tuyama; Gastrodin; MAPKs; Kainic acid; Reactive oxygen species; Epilepsy; ROS; COX-2
Lee YC, Chuang TW, Lin WJ, Hou CW. The Tuyama and Gastrodin Attenuate Excitatory Damage in Kainate Stressed Neuronal Cells. Ann Epilepsy Seizure. 2021;3(1):1011..