Inass A Sadawe1, Amira A Gbaj2, Abdelraouf O Algheryane3, Nisreen H Meiqal1, Salah M Bensaber1, Abdulathim AA Alshoushan4, Halima A Gbaj1, Massaud Salem Maamar5, Anton Hermann6 and Abdul M Gbaj1*
1Department of Medicinal Chemistry, University of Tripoli, Libya
2Novelien School, Tripoli Centre, Libya
3National Commission for DNA Research and Analysis, Libya
4Food and Drug Control Centre (LFDA), Libya
5Department of Zoology, Tripoli University, Libya
6Department of Biosciences, University of Salzburg, Salzburg, Austria
Gambierdiscus spp). The toxin is accumulated and transformed throughout the sea food chain causing many life-threatening neurological problems in Libya and other Mediterranean and North African countries and eventually may cause death. The plant species Echium angustifolium which is locally known as “Hannet Al-Aggrab” contains pyrrolizidine alkaloids, phenolic acid derivatives, flavonoids and other constituents that are known for their numerous biological activities. For treatments of ciguatera fish poisoning the plant has not been studied so far in the Mediterranean and North Africa regions. In the present study, Echium angustifolium aqueous extract was evaluated for its ability to reduce or revoke the effect of ciguatoxins in mice. Molecular docking and in vivo animal studies were performed in order to determine the potential effect of Echium angustifolium aqueous extract against ciguatoxicity. The content of Echium angustifolium extract was evaluated using molecular modeling against ciguatoxin on Sodium (Na+) and Potassium (K+) voltage-gated channels. Hesperetin was found the most active compounds with a Gibbs energy of -8.5 Kcal/mol. For K+ voltage-gated channels was ellagic acid which was the most active compounds with a Gibbs energy of -9.0 Kcal/mol. Our results revealed that Echium angustifolium constituents form hydrogen bonds with active sites of Na+ and K+ channels and protect the mice from ciguatoxin toxicity. We propose that Echium angustifolium constituents can be used to prevent ciguatoxin toxicity. Further chemical synthesis of analogues and in vivo studies will be necessary to substantiate the obtained results.
Ciguatera fish poisoning; Ciguatoxin; Neurotoxin; Molecular modeling; Na+; k+
Sadawe IA, Gbaj AA, Algheryane AO, Meiqal NH, Bensaber SM, Alshoushan AAA, et al. Evaluation of Aerial Parts of Echium angustifolium on Ciguatoxins Toxicity using Molecular Modeling and Albino Mice Models. Ann Clin Toxicol. 2020; 3(1): 1030..