Ann Clin Immunol MicroBiol | Volume 1, Issue 1 | Case Report | Open Access
Fuchao Chen1,2, Tingting Shi1, Quan Zhu1, Lijia Xiao1, Fuyan Wang1, Yunhong Tan3, Kanglin Wan4, Qizhi Luo1* and Ping Yu1
1Department of Immunology, School of Basic Medical Science, Xiangya School of Medicine, Central South University, China
2Department of Immunology, Zunyi Medical College, China
3Clinical Laboratory, Hunan Chest Hospital, China
4National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, China
Background: Mycobacterium tuberculosis (M. tb) infection is a major global threat, the protective effect of traditional vaccine BCG is poor for adults, and therefore, an effective vaccine against this pathogen is urgently required. Base on this background, we determined to explore the new vaccine for prevent the infection by M. tb, so we expressed and purified two M. tb recombinant proteins, Rv1986 and Rv3823c, to investigate their role in induction of immunity.
Methods: Peripheral blood samples were collected from 100 patients diagnosed with TB and admitted to the Hunan Provincial Chest Hospital (Changsha, China) for treatment. The TB diagnosis was confirmed by positive M. tb culture and subsequent biochemical tests. All patients underwent chest radiography. Blood samples were also obtained from 100 healthy individuals and 100 patients with non-TB respiratory diseases. Subjects from both sexes were included and the female to male ratio was 49:51. The age range was from 14 to 81 years with the average age of 42 years. This study was reviewed and approved by the Institutional Ethical Committee of the Xiangya Hospital. Written informed consent was obtained from each participant.
Results: Serum antibodies recognized Rv1986 and Rv3823c in 24% and 32% patients with TB, respectively, in an Enzyme-Linked Immunosorbent Assay (ELISA) using the sera of 100 patients with Tuberculosis (TB) and 200 individuals without TB. Both proteins stimulated the expansion of effector memory T cells and central memory T cells as well as plasma cells in mouse vaccination experiments. Furthermore, Rv1986 induced higher serum interferon-γ level in immunized mice.
Conclusion: The results suggested that Rv1986 and Rv3823c were highly immunogenic antigens and could stimulate humoral and cell-mediated immune responses.
Mycobacterium tuberculosis; Memory T cell; Inflammatory cytokine; ELISA; Specific antibody
Chen F, Shi T, Zhu Q, Xiao L, Wang F, Tan Y, et al. The Role of Mycobacterium tuberculosis Rv1986 and Rv3823c in Stimulating Humoral and Cell-Mediated Immune Responses. Ann Clin Immunol Microbiol. 2018;1(1):1004.