Ann Clin Anesth Res | Volume 6, Issue 1 | Research Article | Open Access

Pharmacological Evaluation of Indigofera argentea for Its Antidiabetic Activity and Ameliorative Potential on End Organ Damage in Streptozotocin-Induced Diabetic Rats

Qaiser Jabeen1*, Mahvish Kiran1, Mushtaq Ahmad1, Shaheen Gul1, Naveed Aslam2 and Hafiz Muhammad Farhan Rasheed1,2

1Department of Pharmacy, The Islamia University of Bahawalpur, Pakistan 2Department of Pharmacy, Drug Testing Laboratory Punjab, Pakistan

*Correspondance to: Qaiser Jabeen 

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Research in drug discovery from medicinal plants involves a multidimensional approach including botanical, phytochemical, biological and molecular techniques. The objective of the study was to evaluate the antidiabetic effects of 70% methanolic extract of Indigofera argentea (Ia.Cr), a plant from Cholistan desert of Pakistan, and its ameliorative potential on end organ damage in streptozotocininduced diabetic rats. Male Wistar albino rats were divided into different groups each comprising of six animals. Diabetes was induced in 12 h fasted animals by injecting streptozotocin (STZ, 60 mg/kg; i.p). Blood glucose levels were checked after 72 h and then two weeks after administration of STZ, the animals having levels >250 mg/dl were considered diabetic and included in further studies. The normal control and diabetic control groups were administered distilled water (4 ml/ kg/day), standard control group was administered metformin (500 mg/kg/day), and the treatment groups were given Ia.Cr at the doses of 30, 100, 300 mg/kg/day; p.o. for 28 days. Blood of animals was drawn by cardiac puncture, sera separated and analyzed. Histological examination of liver, kidney and heart of one representative animal from each group was performed. STZ was found to induce diabetes mellitus in rats and also caused end organ damage that was demonstrated by hepatic markers (increased levels of ALT, AST, ALP & decreased levels of albumin), elevated renal markers (urea, creatinine and uric acid), lipid profile (increased levels of cholesterol, triglycerides, LDL, and decreased levels of HDL) and histological findings. Ia.Cr was found to produce dose-dependent antidiabetic effects and highly significant (P<0.001) results at the dose of 300 mg/kg, as compared to the diabetic control group. The study concludes that Indigofera argentea possesses the antidiabetic effects and prevents the end organ damage caused by STZ.


Indigofera argentea; Anti diabetic activity; Streptozotocin; Hyperlipidemia


Jabeen Q, Kiran M, Ahmad M, Gul S, Aslam N, Rasheed HMF. Pharmacological Evaluation of Indigofera argentea for Its Antidiabetic Activity and Ameliorative Potential on End Organ Damage in Streptozotocin- Induced Diabetic Rats. Ann Clin Anesth Res. 2022; 6(1): 1045..

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