Tzou RD, Hsu YC , Tsai TH, Lee KT , Huang TY and Fan WC
1Department of Surgery, Division of Neurosurgery, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan 2School of Medicine, I-Shou University, Kaohsiung, Taiwan 3Department of Surgery, Division of Neurosurgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan 4Department of Surgery, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan 5Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan 6 Department of Neurosurgery, Changhua Christian Hospital, Changhua, TaiwanFulltext PDF
Objectives: Integrin α3 (ITGA3), which is located on the cell membrane and functions as a cell surface adhesion molecule, is thought to be a potential molecular marker in several cancers. Its expression has also been correlated with metastasis. This study is the first to investigate the biological function of this protein in human Glioblastoma Multiforme (GBM). Materials and Methods: Protein expression of ITGA3 in glioma tissue samples was first measured immunohistochemically and correlated with other clinical parameters. Short interfering RNA (siRNA) was added to different cell lines to block ITGA3 activity and evaluate its effect on proliferation, invasion, adhesion, and migration by assay. Western blot analyses of these cell lines were performed to measure the expression of ITGA3, N-cadherin, and Cyclin D1 signaling molecules. Results: We found that the expression of ITGA3 was high in glioma cells and that its high expression was significantly and independently predicted poor survival in patients with astrocytoma. While ITGA3 siRNA treatment attenuated survival/proliferation, it also inhibited locomotion of GBM8401 and U87-MG cells in vitro, possibly through its inhibition of N-cadherin and Cyclin D1 signaling molecules. This knockdown of ITGA3 improved TMZ-related cytotoxicity in glioma cells. Conclusion: ITGA3 was found to be a potential prognostic biomarker. While ITGA3 may predict the glioma cell progression, it could possibly be targeted to inhibit metastasis activities in established GBM cells.
Integrin α3; ITGA3; GBM; Cell adhesion; Cell locomotion
Tzou RD, Hsu YC, Tsai TH, Lee KT, Huang TY, Fan WC. Integrin Α3 (ITGA3) as a Prognostic Marker and Potential Therapeutic Anti-Metastasis Target in Glioma. Am J Otolaryngol Head Neck Surg. 2022;5(8):1205.