Am J Allergy Asthma Immunol Res | Volume 1, Issue 1 | Research Article | Open Access
Hirokuni Otsuka1,2*, Kuninori Otsuka3 and Kimihiro Okubo4
1Otsuka ENT Clinic, Japan
2Department of Otorhinolaryngology, Nippon Medical School, Musashikosugi Hospital, Japan
3Department of Otorhinolaryngology, General Tokyo Hospital, Japan
4Department of Otorhinolaryngology and Head and Neck Surgery, Nippon Medical School, Japan
*Correspondance to: Hirokuni OtsukaFulltext PDF
Background: There are several papers that suggest the involvement of MCT increased in nasal surface of allergic rhinitis but not MCTC in nasal submucosa.
We studied whether MCTC in nasal submucosa could directly respond with specific antigen and stem cell factors (SCF) enhance the sensitivity of MCTC.
Methods: Using nasal subglandular submucosa and nasal surface scrapings of the inferior turbinate from patients sensitized solely to mite, we studied allergen induced histamine and leukotriene release, and whether allergen induced both mediators release from nasal subglandular submucosa was enhanced pretreatment with SCF.
Results: Histamine and leukotriene release from nasal scrapings were increased with mite allergen extract (from 2.2 ng PN/ml to 2.2 μg PN/ml). By contrast, those from fragments of nasal submucosa of same patients were very low under the same concentration of mite extract. However, when fragments of nasal submucosa were pre-treated with SCF, mite antigen 0.22 μg PN/ml induced– histamine and leukotriene release was significantly enhanced.
Conclusion: These findings suggested that MCTC in the submucosa mucosa were lower sensitive with specific antigen than MCT in nasal epithelium. It might be there they had a phenotype dependent on exogenouse SCF derived from epithelial cell to enhance allergen-induced release of histamine and leukotriene. Mast cells in nasal epithelium are constitutively more sensitive to allergen challenge, perhaps because of ongoing exposure to epithelial-derived SCF.
Mast cell; Nasal submucosa; Nasal surface; Stem cell factor; Histamine release; Leukotriene release; Mite allergen
Otsuka H, Otsuka K, Okubo K. Reactivity to Specific Antigen of Submucosa Mast Cells in Allergic Rhinitis was Very Low but Enhanced by Pretreated with Stem Cell Factor. Am J Allergy Asthma Immunol Res. 2022;1(1): 1003..