Ann Plast Reconstr Surg | Volume 3, Issue 4 | Research Article | Open Access
Xiaohe Luo1,2, Yimei Wang3, Gang Chen1*, Wei Wang1*, Wenjin Wang1 and Wei Ding1
1Department of Plastic and Reconstructive Surgery, Shanghai Jiao Tong University School of Medicine, China 2Department of Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China 3Department of Plastic Surgery, First Affiliated Hospital of Nanchang University, China
Fulltext PDFObjective: To investigate the effect of Adipose-Derived Stem Cells (ADSCs) on the proliferation and dedifferentiation of Schwann Cells (SCs) at the early stage of Wallerian Degeneration (WD) by constructing an in vitro rat model. Methods: After extracting subcutaneous adipose tissue from the groin of Sprague-Dawley (SD) rats under sterile conditions, ADSCs were isolated and cultured in vitro. ADSC morphology and growth status were observed by inverted microscopy, and surface markers were examined by flow cytometry. WD in an in vitro rat model was created using sciatic nerves, which were divided randomly into experimental and control groups. In the experimental group, sciatic nerves and ADSCs were co-cultured without contact, while sciatic nerves were cultured alone in the control group. Real time PCR was conducted to detect p75NTR and MPZ mRNA expression at different time points (0 h, 24 h, and 72 h), and p75NTR and MPZ protein expression was detected after in vitro culture for 72 h by Western blotting. SC proliferation and apoptosis in both groups were examined at different time points (24 h, 48 h, and 72 h) by Immunohistochemistry (IHC) and TUNEL staining, respectively. Results: Flow cytometry showed that CD90 and CD105 were highly expressed in ADSCs, but CD34 was barely expressed. After 72 h of culture, the mRNA expression of the dedifferentiation marker p75NTR was greatly increased, and a significant growth trend was found in the experimental group (P<0.05). The mRNA expression of the differentiation marker MPZ was gradually reduced, with a more obvious decreasing trend in the experimental group (P<0.001). In addition, compared with that in the control group, p75NTR protein expression was significantly higher after 72 h of co-culture (P<0.05), and MPZ expression was significantly lower (P<0.001). In addition, Ki67 expression was significantly higher in the experimental group than in the control group according to IHC, and the apoptosis rate was lower in the experimental group according to TUNEL staining. Conclusion: ADSCs might promote SC proliferation and dedifferentiation in WD and inhibit apoptosis.
Adipose-derived stem cells; Schwann cells; Wallerian degeneration; Proliferation; Dedifferentiation
Luo X, Wang Y, Chen G, Wang W, Wang W, Ding W. Effect of Adipose-derived Stem Cells on Proliferation and Dedifferentiation in Schwann Cells in the Early Stage of Wallerian Degeneration. Ann Plast Reconstr Surg. 2019; 3(4): 1041..