Wesam Hassanein A1, Hanan Elsayed M2, Rehab El-Sokkary H2* and Asmaa Mekky F1
1Department of Microbiology, Zagazig University, Egypt
2Department of Medical Microbiology and Immunology, Zagazig University, Egypt
Background: The prevalence of bacteria in a neonatal intensive care unit represents a major health problem. We aimed to study the bacteria isolated from infected neonates and bacterial colonization on the environmental surfaces, identify their susceptibility pattern, detect their virulence factors and describe the effect of cefotaxime on the surface adherence properties for the most resistant bacteria.
Methods: Isolates were collected from infected neonates and environmental surfaces, identified and antibiotic susceptibility was determined and were screened for virulence factors and biofilm formation.
Results: A total of 100 clinical specimens and 100 swabs from environmental surfaces were collected. Forty four clinical specimens showed positive bacterial culture, 34 (77.3%) from blood and 10 (22.7%) from ETT aspirate. Fifty one swab cultures from environmental surfaces showed positive bacterial culture. Coagulase-Negative Staphylococci (CoNS) were more abundant (42.7%) followed by S. aureus (21.3%), Pseudomonas spp. (10.7%). K.Pneumoniae and S. aureus isolated from ETT aspirate were most resistant to cefotaxime. Adhesion force was visualized as gradual increase in crystal violet color of ETT specimens and bacterial aggregates in scanning electron microscopy. Conclusions: S. aureus showed more adherence on the surface of ETT specimen than K. Pneumoniae. Treatment of ETT specimens with Minimal Inhibitory Concentrations (MIC) prevents the adhesion of the pathogenic K. pneumonia and S. aureus.
NICU; Biofilm; Infection control; Colonization; Cefotaxime; Adhesion
Wesam Hassanein A, Hanan Elsayed M, Rehab El-Sokkary H, Asmaa Mekky F. Bacteria Isolated from a Neonatal Intensive Care Unit at an Egyptian University Hospital: Antibiotic Susceptibility and Virulence Factors. Ann Microbiol Immunol. 2019; 2(1): 1010.