Tariq U, Sadiq Butt M, Hameed A
National Institute of Food Science & Technology, University of Agriculture, PakistanFulltext PDF
Introduction: Lead (Pb) exposure is a worldwide unavoidable environmental problem because of its presence in various useable appliances causing severe health problems. Different minerals like Zinc (Zn) and Calcium (Ca) are known to exert competitive behavior with Pb because of structural analogy thus hinder Pb absorption in blood.
Objective: To determine individual as well as synergistic potential of Zn and Ca in inhibition of Pb induced adverse impact on rat hematology and antioxidant enzymes.
Methodology: In controlled environment, Sprague dawley rats received Lead acetate 20 mg/kg of Body Weight (bw), zinc sulphate 20 mg/kg bw and calcium carbonate 7500 mg/kg bw with different combinations via nasogastric tube feeding for 35 days. Blood samples collected from rats were tested for complete blood count and antioxidant enzymes in blood.
Results: Lead exposure significantly decreased the red blood cells, hemoglobin, hematocrit, monocytes, granulocytes, platelet count (P<0.01) white blood cells and red blood cell distribution width (P<0.05). Antioxidant enzymes including superoxide dismutase and catalase were also found significantly reduced (P<0.01) after Pb exposure. These alterations in hematological and antioxidant enzymes concentrations were lessened by Zn, Ca and Zn+Ca supplementation, with Zn being more effective than others.
Conclusion: Results of this study clearly shows that Zn and Ca either alone or in combination possess ameliorative potential against the hallmarks of Pb induced oxidative stress but Zn alone showed greater impact than Ca and Zn+Ca supplementation. So it can be inferred that Zn and Ca supplementation could act as potential preventive strategy to hinder Pb induced hematological alteration and oxidative stress.
Hematology; Lead toxicity; Synergistic; Zinc; Calcium; Oxidative stress
Tariq U, Sadiq Butt M, Hameed A. Probing the Individual and Synergistic Effect of Calcium and Zinc against Lead Induced Hematological Alterations and Oxidative Stress. Ann Complement Altern Med. 2018; 1(1): 1003.