Ann Surg Case Rep | Volume 7, Issue 2 | Research Article | Open Access
Qianman B1#, Jiasharete T2#, Wupuer A1#, Jialihasi A3, Shawutali N4, Buranbai D5, Yin Z6, Maimaiti X6, Alimujiang A6, Xu X6, Tieliuhan T7, Ydyrys A8 and Jielile J1*
1Department of Orthopedics & Ankle Surgery, The Sixth Teaching Hospital of Xinjiang Medical University, China
2Department of Clinical Medicine, Capital Medical University, China
3Department of Orthopedics, Almaty Medical Center HAK, Kazakhstan
4Department of Pediatric Surgery, The People's Hospital of Xinjiang Uygur Autonomous Region, China
5Brain Function Analysis & Digital Medicine Research Institute, Showa University, Japan
6Department of Orthopedics Centre, The First Teaching Hospital of Xinjiang Medical University, China
7Department of Central Institutes of Health, Kupu Township, China
8Al-Farabi Kazakh National University, Biomedical Research Center, Kazakhstan
#The authors contributed equally to this study
*Correspondance to: Jiasharete Jielile
Fulltext PDFBackground: The traditional medicine using Horse Oil Percutaneous Permeation (HOPP) has been recommended for the treatment of ankylosis. In this study, using a quantitative proteomics approach, we investigated the therapeutic mechanism of HOPP in a rat ankylosis model. Methods: Sixty Sprague-Dawley (SD) rats with ankle joint ankylosis were randomized to a treatment group that received HOPP (n=30) or a control group (n=30). Synovial tissue samples from the ankle joint were collected at 7, 14, and 21 days in each group. Protein levels were assessed using isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Differentially expressed proteins subjected to bioinformatics analysis. Results: Among 1,729 proteins identified in synovial tissue samples, 200 proteins were differentially expressed across three time points between the HOPP treatment and control groups. Further analysis revealed that the differentially expressed proteins are involved in energy metabolism, calcium metabolism, myosin proteins, and mitogen-activated protein kinase signaling, and thus, these may represent the major mechanisms of the therapeutic effects of HOPP. Conclusion: The results provide mechanistic insight into the improved joint function achieved with HOPP treatment, and thus, could be useful in the development of improved clinical treatments for anchylosis.
Ankylosis; Arthrofibrosis; Traditional medicine; horse oil; iTRAQ; Proteomics; Therapeutic mechanism
Qianman B, Jiasharete T, Wupuer A, Jialihasi A, Shawutali N, Buranbai D, et al. Quantitative Proteomic Analysis Provides Insight into the Therapeutic Mechanism of Horse Oil Percutaneous Permeation Treatment in a Rat Ankylosis Model. Ann Surg Case Rep. 2024; 7(2): 1090..