Yichun Yang, Zhongxiang Jiang and Zheng Jiang*
Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, ChinaFulltext PDF
The aim of our study was to elucidate the mechanism of action of the Insulin-like Growth Factor 2 mRNA-Binding Protein 1 (IGF2BP1) on the phenotype of the Hepatocellular Carcinoma (HCC). To gain insight into the mechanism of action of the IGF2BP1 on HCC, the IGF2BP1 shRNA sequences were transfected into HCC cells. The HCC phenotypes were transduced into HCC cells. The effects of IGF2BP1 on HCC were investigated through assays of cellular proliferation, invasion and migration. Next, we screened for possible molecular mechanisms from the TCGA database, and found a target gene Chromobox 6 (CBX6). The RIP-qPCR was used to detect the binding of the IGF2BP1 and CBX6 mRNA. We adjusted the attenuation of the mRNA of the CBX6 using quantitative Real-Time PCR (qRT-PCR). Furthermore, rescue experiments were used to further demonstrate that CBX6 is a
target of IGF2BP1. The IGF2BP1 was upregulated in HCC, and promoted the malignant phenotype of HCC cell lines. Further exploration indicated that the IGF2BP1 binds directly to the CBX6 and inhibits its mRNA attenuation, suggesting that the IGF2BP1 impacts CBX6 mRNA stability. Thus, it can be concluded that the IGF2BP1 facilitated the HCC phenotypes by promoting the CBX6 mRNA stability.
IGF2BP1; Hepatocellular carcinoma; RNA binding proteins; CBX6; mRNA stability
Yang Y, Jiang Z, Jiang Z. IGF2BP1 Promotes Proliferation and Metastasis of Liver Cancer by Regulating CBX6
mRNA Stability. J Gastroenterol Hepatol Endosc. 2021; 6(4): 1104..