J Dent Oral Biol | Volume 5, Issue 3 | Review Article | Open Access

Variation in Osteoblast Retention Ability of Titanium Surfaces with Different Topographies

Mitsunori Uno^1*, Ryotaro Ozawa¹, Kosuke Hamajima¹, Juri Saruta¹, Hajime Ishigami² and
Takahiro Ogawa¹

¹Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, UCLA School of
Dentistry, Los Angeles, USA
²Department of Prosthodontics, Division of Oral Functional Science and Rehabilitation, Asahi University School of
Dentistry, Gifu, Japan

^*Correspondance to: Mitsunori Uno 

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Abstract

The ability of titanium surfaces to retain osteogenic cells is critical for osseointegration. In particular, the cell-retention ability under exogenous force holds a key to successful osseointegration in immediately or early loaded implants. However, it is unknown which surfaces are better than the others in terms of their cell-retention ability, how much is the potential difference among different surfaces, and what topographical variables determine the ability. Rat bone marrow- derived osteoblasts were cultured on titanium disks with four different surface topographies: machined, sandblasted, acid-etched, and sandblasted + acid-etched surfaces. After a 24-h incubation, cells were detached using vibrational force combined with enzymatic protein degradation (trypsinization). The cell-retention ability was greater in the order of sandblasted + acid-etched surface, sandblasted surface, acid-etched surface, and machined surface. There was a significant linear correlation between the cell-retention ability and the degree of surface roughness. Sa (average roughness) and Sdr (developed interfacial area ratio) were highly correlated with the cell-retention ability. However, the number of cells recruited during a 24-h incubation was negatively correlated with the degree of surface roughness, with the sandblasted + acid-etched surface recruiting the least number of cells. In conclusion, the rougher the titanium surfaces, the stronger their cell-retention ability. Sdr was the most effective topographical determinant to predict both cell-retention and recruitment ability.

Keywords:

Sdr (Developed interfacial area ratio); Cell-Recruitment ability; Cell-Retention ability; Mechanical detachment test

Citation:

Uno M, Ozawa R, Hamajima K, Saruta J, Ishigami H, Ogawa T. Variation in Osteoblast Retention Ability of Titanium Surfaces with Different Topographies. J Dent Oral Biol. 2020; 5(3): 1169. ISSN: 2475-5680.