J Dent Oral Biol | Volume 3, Issue 7 | Research Article | Open Access

Therapeutic Potential of Intra-Articular Injection of Bone Marrow Mesenchymal Stem Cells on Tempromandibular Joints' Induced Osteoarthritis. An Experimental Study

Mazen Tharwat Abou Elkhier¹, Nesreen Nabil Mohamed² and Mohamed I. Mourad^3*

¹Department of Oral Biology, Mansoura University, Egypt
²Department of Dentistry, Mansoura University, Egypt
³Department of Oral Pathology, Mansoura University, Egypt

^*Correspondance to: Mohamed I. Mourad 

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Abstract

Background: The advancement of regenerative medicine and innovative stem cell technology offers a unique opportunity to treat Osteoarthritis (OA). Objectives: To evaluate effect of Bone Marrow Mesenchymal Stem Cells (BMMSCs) on healing of Temporomandibular Joints' (TMJ) in rats with induced rheumatoid arthritis. Methods: Thirty rats were randomly divided into three equal groups; ten for each. Group I: negative control. Group II: positive control and was subjected to induction of adjuvant arthritis CFA (Sigma Aldrich, St. Louis, Missouri, United States) bilaterally into the TMJ on the start of experiment. Groups III (n=10) was handled as those in group II, but after 10 days, TMJ was injected locally with 1 × 106 cell/ml BMMSCs. Animals of all groups were euthanized three weeks after the start of experiment. Results: Group I revealed normal characters of the TMJ, Group II showed thickening of disc, thinning of cartilage, disordered bone trabeculae and decreased, disarranged collagen fibers after 2 weeks, while after 3 weeks it showed furthermore aggravated effects with adhesion between disc and condylar cartilage. Group III showed slight widening of marrow spaces, almost normal thickness of disc and condylar cartilage, near normal arrangement of bone trabeculae and regenerated collagen fibers.
Conclusion: These results suggest that BMMSCs may represent a novel and effective therapy for treatment of rheumatoid arthritis.

Keywords:

Arthritis; Bone marrow stem cells; Osteoarthritis; TMJ

Citation:

Abou Elkhier MT, Mohamed NN, Mourad MI. Therapeutic Potential of Intra-Articular Injection of Bone Marrow Mesenchymal Stem Cells on Tempromandibular Joints' Induced Osteoarthritis. An Experimental Study. J Dent Oral Biol. 2018; 3(7): 1150.