J Clin Nephrol Kidney Dis | Volume 4, Issue 2 | Review Article | Open Access

Comparison of Colistin Susceptibility Testing by VITEK 2 Compact and Broth Microdilution Method for Carbapenem Resistant Isolates in a Tertiary Diagnostic Centre

Shaheen Shaikh^* and Heeral Pandya

Suburban Diagnostics, Mumbai, India

^*Correspondance to: Shaheen Shaikh 

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Abstract

A study was undertaken to compare colistin susceptibility using BMD and Vitek in carbapenem resistant gram negative isolates to evaluate the discrepancies and further course of action. The Broth Microdilution (BMD) technique is reliable and is easy to use method for determining the MIC of Colistin. The results correlated with Vitek system except for 2 isolates which showed very major errors which indicates that in case of resistance to Colistin by Vitek, broth dilution method must be used for correlation and to recheck the result. Also in case of Vitek system showing susceptibility to Colistin, we can safely report those isolates without doing micro broth dilution as we did not encounter any isolates which gave susceptible on Vitek and resistant on micro broth dilution method.

Keywords:

Colistin; Vitek; Broth microdilution; MIC

Citation:

Shaikh S, Pandya H. Comparison of Colistin Susceptibility Testing by VITEK 2 Compact and Broth Microdilution Method for Carbapenem Resistant Isolates in a Tertiary Diagnostic Centre. J Clin Nephrol Kidney Dis. 2019; 4(2): 1024.