Case Report

The Basic Knowledge of Varicella Zoster Virus (VZV) Molecular Genetic Testing

Vanda Boštíková1, Radek Sleha1*, Sylva Janovska1, Miloslav Salavec2, Roman Chlíbek1, Miroslav Špliňo1 and Pavel Boštík1,3
1Department of Military Health Sciences, University of Defence, Czech Republic,
2Department of Dermatology, Charles University Medical School and University Hospital, Czech Republic
3Department of Infectious Diseases, Charles University Medical School and University Hospital, Czech Republic

*Corresponding author: Radek Sleha, Department of Military Health Sciences, University of Defence, Třebešská 1575, 500 01 Hradec Králové, Czech Republic

Published: 02 Feb, 2017
Cite this article as: Boštíková V, Sleha R, Janovska S, Salavec M, Chlíbek R, Špliňo M, et al. The Basic Knowledge of Varicella Zoster Virus (VZV) Molecular Genetic Testing. Ann Infect Dis Epidemiol. 2017; 2(1): 1008.


VZV is unique among the herpes viruses in its transmission by inhalation of aerosolized virus particles. Infection via the respiratory route increases the opportunity for rapid spread of the virus through susceptible populations and exposure of those already latently infected.
The genotyping of VZV helps to better understand differences in properties of individual wild type and/or vaccine VZV strains; inform about pathogenic potential of individual VZV strains and help to detect mutations in functionally important gene and analyze their effects on phenotype.
Keywords: Varicella-zoster virus; Herpes zoster; Molecular genetic; Genotyping


Varicella zoster virus (VZV) is a highly contagious human pathogen. It is easily transmitted within families school classmates and other groups by airborne particles and droplets in exhaled air, or, on a closer contact, by the fluid from blisters or sores. Virus can be also transmitted indirectly by contact with clothing and other items exposed to fresh drainage from open sores. Patients are contagious up to five days before and five days after the appearance of their rash. When sores have crusted over, the patient is usually no longer contagious [1,2].
VZV causes varicella (chickenpox) on primary infection, during which a lifelong latent infection is established [3]. Chickenpox is characterized by one to two days of low grade fever, general weakness, prodromal malaise, pharyngitis, rhinitis and a rash, which is often the first sign of the disease [4,5]. Rarely, patient may experience the VZV infection and disease without rash. The rash of chickenpox is typically very itchy Shinjoh and Takahashi et al. [6] and develops in crops with raised red spots appearing first, progressing to blisters that burst and create open sores, before crusting over [7,8]. The process usually starts on the scalp, then involves the trunk, and finally spreads centrifugally to the arms and legs. Less commonly the lesions can be found in the mucosa of the oropharynx and vagina [9,10]. Any area of skin that is irritated (eczema, diaper rash, sunburn) could be seriously affected by the rash of VZV Kaushik et al. [11]. In adults, complications are more frequent in pregnant women, with negative consequences for the fetus and the newborns, and in immunocompromised people, with an increased risk of mortality [12-17].
A lifelong latent infection is established during the first exposure and can reactivate, typically after age fifty, to cause herpes zoster (HZ, shingles) [18-20]. In these cases lesions are localized and painful, often involving the trunk and following an area of dermatime. Herpes zoster occurs frequently in adults, but is not uncommon in immunocompromised and even normal children [21- 23]. Some patients develop serious pain that lasts for months after the rash has healed, which is called post herpetic neuralgia (PHN) Gilden et al. [24].
Virus can be transmitted to susceptible persons suffering from either disease condition, although zoster carries a significantly lower risk of transmission. Varicella cases arising through transmission from zoster patients result in the reintroduction of strains, which first infected the individual decades earlier, and, as such, likely contribute to the genetic stability of VZV [25,26].

VZV Epidemiology

The epidemiology of VZV infection varies geographically [27-29]. Varicella displays marked seasonality (peak in spring) in temperate climates, and infection is nearly ubiquitous by the age of 20. For no clear reasons, seasonality does not occur in tropical countries, and larger proportion of people enter adulthood uninfected by VZV [30,31]. In addition, several studies have demonstrated a distinctive geographic distribution of the major VZV genotypes aligning with cool versus warm climate regions of the globe [32,33]. It is unclear whether the strain distribution is actually driven by climate or/and other factors, such as immigration patterns Loparev et al. [30], Loparev et al. [34].
In Czech Republic the incidence of varicella ranges between 35,000 to 40,000 cases/year during last ten years (Epidat system, There were three incidence peaks in 1998, 2004 and 2007 years, when the incidence reached 50,000 cases/year. New cases always occur from January to June with a peak typically in May. An average of 6,000 cases of zoster annually during the last decade has been reported via Epidat system for Czech population. Czech women are affected about 1.4 times more often than men and the peak incidence of HZ is around 70 years of age.
VZV exhibits tropism for neural tissues and therefore neurologic complications, which can occur before or after the acute infection Sauerbrei and Wutzler et al. [35], are typical for VZV infection, Cerebellar ataxia is one possible benign VZV complication that is thought to be due to post infection demyelination. It is estimated to occur in 1 in 4000 cases among those younger than 15 years old. Ataxia, vomiting, altered speech, fever, vertigo and tremor are common symptoms, but resolution usually occurs within 2 to 4 weeks. Lee et al. [25], [36,37].
More serious complication of VZV is encephalitis, which occurs in 0.1 to 0.2 % of patients. Mortality has been estimated to range between 5 to 20 % and sequelae have been described in 15 % of survivors Saez-Llorens et al. [38].
Reye’s syndrome is described in association with varicella, often with concomitant use of aspirin in children younger than 5 years. It begins in late stages of varicella with vomiting, restlessness, irritability, and progressive decrease in the level of consciousness, associated with progressive cerebral edema Malavige et al. [2] Immunocompromised patients contracting VZV are at risk of more severe course of disease. They may develop severe skin eruptions with or without hemorrhage and healing even of the "typical" VZV cutaneous lesions takes three times longer than in the general population. These patients may also develop high fever and the virus also more often spreads to visceral organs causing hepatitis, pneumonitis, pancreatitis, and encephalitis. Bacterial super infections (Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus) of the primary virus leasions, leading to bacteremia can develop.
Antiviral therapy significantly reduces morbidity and can reduce mortality. Rarely, varicella in early pregnancy (first 20 weeks) may result in congenital varicella syndrome (CVS). CVS occurs in 2% of infections and is characterized by unusual cutaneous defects, cicatricial skin scars, and atrophy of the extremities. The patients often have microcephaly, cortical atrophy, seizures, mental retardation, chorioretinitis, microphthalmia, or cataracts. Newborns, whose mothers develop varicella close to term, are in risk of neonatal varicella. These babies can be born with varicella lesions or develop lesions after birth, but they typically do not develop serious complications [39-41].
In addition herpes zoster can lead to a wide spectrum of serious health complications [42,43]. Zoster can affect the cornea causing iridocyclitis with secondary glaucoma (herpes zoster ophthalmicus) Eskiizmir et al. [19]. Postherpetic neuralgia may occur in as many as 25 to 50 % of patients older than 50 years. Almost 50 % of persons older than 50 suffer debilitating pain for more than 1 month [44,45]. Meningoencephalitis and encephalitis are described in patients with zoster as well Vandercam et al. [46].
Immunocompromised patients can experience a more severe form of the disease Tavazzi et al. [41]. Lesions form for up to 2 weeks after infection and scabbing occurs until 3 to 4 weeks into the course of the disease. Patients with lympho proliferative malignancies are at risk of cutaneous dissemination and visceral involvement, including pneumonitis, hepatitis, and meningoencephalitis Sutradhar et al. [47]. Eight to eleven percent of patients with HIV are infected with VZV. These patients can develop chronic herpes zoster, with formation of new lesions without the healing of the already existing ones.
Although rapid spread and a low mutation rate could both explain the low level of genetic diversity of VZV, the geographical distribution and relatedness of strains does suggest that the spread of VZV subsequent to the major human migrations has made a strong contribution to its geographic epidemiology Loparev et al. [30]. Rapid spread is possible, because VZV, uniquely among herpes viruses, is transmitted by aerosolization of virus, resulting in airborne epidemic infections. Geographic spread may be particularly rapid in warmer regions, where varicella occurs on average 10 years later in life. In this scenario, the disease is transmitted between individuals who are more mobile than the children, who otherwise account for the majority of infectious cases in more temperate climates Loparev et al. [30], Loparev et al. [48], Loparev et al. [34].

VZV Genome

The virus has icosahedral symmetry and contains centrally located linear double-stranded DNA molecule 124,884 base pairs (bp) in length with surrounding envelope. The VZV genome has been considered to be stable [49,50]. The variability between individual strains is only ~ 0.1 % and is manifested as single nucleotide polymorphisms (SNP) [51,52]. The genome consists of a unique long region (UL, about 105,000 bp) flanked by an inverted repeat regions (terminal repeat long - TRL, internal repeat long -IRL) and unique short region (US, 5,232 bp) flanked by internal repeat (terminal repeat short -TRS and inverted repeat short -IRS, each 7,319 bp) [53-57].

Basic Molucular Genetic Analysis of VZV Genome

Molecular genetic analysis of VZV strains greatly contributes to the characterization of VZV genotypic groups. Various methods have been utilized and reported for identifying and genotyping VZV strains LaRussa et al. [58], LaRussa et al. [8], Campsall et al. [59], Loparev et al. [30]., Loparev et al. [34], Nagel et al. [60]. Early VZV typing efforts relied on DNA restriction fragment length polymorphism analysis (RFLP), an approach that was sufficiently powerful to demonstrate high degree of sequence conservation among VZV strains. Nevertheless, some intra-strain variation among wild-type isolates of VZV was observed Faga et al. [61], Norberg et al. [55], Parker et al. [62]. VZV DNA restriction fragment length polymorphism (RFLP) has been used to distinguish most wild-type VZV isolates from the Oka vaccine strain, using SNP located in ORF 38 (PstI site in many wild-type strains), ORF 54 (BglI site in Oka) LaRussa et al. [58], LaRussa et al. [8], Loparev et al. [30] and ORF 62 (SmaI site) Li and Zhu et al. [63].
Analysis utilizing this method also determined that wild type VZV strains isolated in the US and Japan have distinctive PstI and BglI RFLP profiles. Japanese isolates are PstI+, BglI+ or PstI-, BglI+, while most isolates from the US, UK, Europe, and eastern Australia isolates are PstI+, BglI- [64,65], Loparev et al. [34]. BglI+ strains, apart from those isolated in Japan, are common in tropical regions such as equatorial Africa, India, Bangladesh, China, Central America and northern Australia [36,37], Liu et al. [66]. An unusual PstI-, BglIVZV strain was reported in Australia Loparev et al. [48], a strain that could represent either recombination between the dominant genotypes or point mutation in the ORF 38 locus.
The majority of already published genotyping efforts were directed to multi-SNP analysis and different SNPs were picked up by different researchers Carr et al. [49], Peters et al. [67], [33,34]. The method developed by Loparev et al, which utilizes the detection of SNPs in ORF 22, differentiated three major VZV genotypes - E (European), J (Japanese), and M (mosaic) - utilising only one amplicon (ORF 22) approach Loparev et al. [54], Loparev et al. [48], Loparev et al. [34], MacNail et al. [68], Norberg et al. [55], Peters et al. [67]. Four SNPs in ORF 22 are able to discriminate also between fpour moyaic strains, named M1, M2, M3 and M4. Studies have shown that strains of the M genotype prevailed in tropical regions, whereas E strains dominated in temperate longitudes and J genotype strains were the dominant genotype in Japan. In later studies, this approach was combined with PCR amplification of the ORF 21, ORF 22 and ORF 50 Loparev et al. [34]. Thus utilization of PCR amplifications of the ORF 21 502 bp target region, ORF 22 447 bp target region and ORF 50 514 bp target region led to further idenitification of the E1 and E2 genotypes.
Strains of the genotype E1 are usually more common in Europe and Australia than E2 strains Loparev et al. [48], Loparev et al. [34]. One hypothesis speculated Norberg et al. [55] that genotype E2 strains may have arisen more than a century ago in remote European colonies such as Australia and New Zealand through recombination events between E1 and tropical genotype M strains and effectively competed with the original tropical and imported E1 strains, establishing themselves in the population Loparev et al. [34]. Recombination events between E1 and J strains could also play a role in the appearance of genotype E2 strains. Alternatively, E2 strain might have emerged in Europe Loparev et al. [54] in those countries with E1 and M strains already in circulation, spreading afterwards to Australia and New Zealand. Recombinant variants of E2 could have been also selected by temperate climates to which increasing numbers of people migrated from warmer regions.


The easiest way to discover even the least predicted variations in the VZV genome is undoubtedly possible only by full genome sequencing approach. However, this approach is relatively expensive and time consuming, which makes it unpractical especially for clinical laboratories. Furthermore, a relatively large quantity of experimental material is needed, which is not always available. This makes the approach of genotyping selected short amplicons with their subsequent bioinformatic analyses a valid and practical method for the epidemiological studies. These are important for tracking changes in VZV epidemiology, with increasing significance especially now during the time of introduction of new vaccination programes both for varicella and zoster in European countries.


  1. Jackowska T, Dziadek J, Wasilewski R, Wasik M. Safety and efficacy of varicella vaccine in healthy children-one year follow up. Med Wieku Rozwoj. 2008; 12: 653-659.
  2. Malavige GN, Jones L, Kamaladasa SD, Wijewickrama A, Seneviratne SL, Black AP, et al. Viral load, clinical disease severity and cellular immune responses in primary varicella zoster virus infection in Sri Lanka. PLoS One. 2008; 3: e3789.
  3. Arvin AM. Varicella-zoster virus: overview and clinical manifestations. Semin Dermatol. 1996; 15: 4-7.
  4. Hambleton S. The role of vaccination in prevention of VZV disease in the immunocompromised child. Clin Adv Hemato Oncol. 2009; 7: 159-161.
  5. Katzoli P, Sakellaris G, Ergazaki M, Charissis G, Spandidos DA, Sourvinos G. Detection of herpes viruses in children with acute appendicitis. J Clin Virol. 2009; 44: 282-6.
  6. Shinjoh M, Takahashi T. Varicella zoster exposure on paediatric wards between 2000 and 2007: safe and effective post-exposure prophylaxis with oral aciclovir. J Hosp Infect. 2009; 72: 163-168.
  7. Beby-Defaux A, Brabant S, Chatellier D, Bourgoin A, Robert R, Ruckes T, et al. Disseminated varicella with multiorgan failure in an immunocompetent adult. J Med Virol. 2009; 81: 747-749.
  8. LaRussa P, Steinberg SP, Shapiro E, Vazquez M, Gershon AA. Viral strain identification in varicella vaccinees with disseminated rashes. Pediatr Infect Dis J. 2000; 19:1037-1039.
  9. Grossberg R, Harpaz R, Rubtcova E, Loparev V, Seward JF, Schmid DS. Secondary transmission of varicella vaccine virus in a chronic care facility for children. J Pediatr. 2006; 148: 842-844.
  10. Habib AA, Gilden D, Schmid DS, Safdieh JE. Varicella zoster virus meningitis with hypoglycorrhachia in the absence of rash in an immunocompetent woman. J Neurovirol. 2009; 15: 206-208.
  11. Kaushik KS, Lahiri KK, Kapila K, Kumar S, Gupta RM, Karade S. Differentiation of wild-type varicella-zoster strains from India and the Oka vaccine strain using a VZV open reading frame - 62 based PCR-RFLP technique. Braz J Infect Dis. 2008; 12: 313-315.
  12. Dayan GH, Panero MS, Debbag R, Urquiza A, Molina M, Prieto S, et al. Varicella seroprevalence and molecular epidemiology of varicella-zoster virus in Argentina. J Clin Microbiol. 2004; 42: 5698-5704.
  13. Gabutti G, Rota MC, Guido M, De Donno A, Bella A, Degli Atti ML, et al. The epidemiology of Varicella Zoster Virus infection in Italy. BMC Public Health. 2008; 27; 8: 372.
  14. Burgoon MP, Cohrs RJ, Bennett JL, Anderson SW, Ritchie AM, Cepok S, et al. Varicella zoster virus is not a disease-relevant antigen in multiple sclerosis. Ann Neurol. 2009; 65: 474-479.
  15. Pour L, Adam Z, Buresova L, Krejci M, Krivanova A, Sandecka V,et al. Varicella-zoster virus prophylaxis with low-dose acyclovir in patients with multiple myeloma treated with bortezomib. Clin Lymphoma Myeloma. 2009; 9: 151-153.
  16. Rodríguez-Violante M, Ordoñez G, Bermudez JR, Sotelo J, Corona T. Association of a history of varicella virus infection with multiple sclerosis. Clin Neurol Neurosurg. 2009; 111: 54-56.
  17. Sutradhar SC, Wang WW, Schlienger K, Stek JE, Xu J, Chan IS, et al. Comparison of the levels of immunogenicity and safety of Zostavax in adults 50 to 59 years old and in adults 60 years old or older. Clin Vaccine Immunol. 2009; 16: 646-652.
  18. Capron J, Steichen O. Disseminated HZ in an elderly patient. Infection. 2009; 37: 179-180.
  19. Eskiizmir G, Uz U, Taşkiran E, Unlü H. Herpes zoster oticus associated with varicella zoster virus encephalitis. Laryngoscope. 2009; 119: 628-630.
  20. Harbecke R, Oxman M N, Arnold BA, Ip C, Johnson GR, Levin M J, et al. Shingles prevention study group. J Med Virol. 2009; 81: 1310-1322.
  21. Lu PJ, Euler GL, Jumaan AO, Harpaz R. Herpes zoster vaccination among adults aged 60 years or older in the United States, 2007: uptake of the first new vaccine to target seniors. Vaccine. 2009; 27: 882-887.
  22. Johnson RW, Wasner G, Saddier P, Baron R. Herpes zoster and postherpetic neuralgia: optimizing management in the elderly patient. Drugs Aging. 2008; 25: 991-1006.
  23. Sampathkumar P, Drage LA, Martin DP. Herpes zoster (shingles) and postherpetic neuralgia. Mayo Clin Proc. 2009; 84: 274-280.
  24. Gilden D, Nagel MA, Ransohoff RM, Cohrs RJ, Mahalingam R, Tanabe JL. Recurrent varicella zoster virus myelopathy. J Neurol Sci. 2009; 276: 196-198.
  25. Lee BW. Review of varicella zoster seroepidemiology in India and Southeast Asia. Trop Med Int Health. 1998; 3: 886-890.
  26. Levin MJ, DeBiasi RL, Bostik V, Schmid DS. Herpes zoster with skin lesions and meningitis caused by 2 different genotypes of the Oka varicella-zoster virus vaccine. J Infect Dis. 2008; 198: 1444-1447.
  27. LaRussa PS, Gershon AA. Biologic and geographic differences between vaccine and clinical VZV isolates. Arch Virol Suppl. 2001; 41-48.
  28. Barrett-Muir W, Scott FT, Aaby PJ, John P, Matondo QL, Chaudhry M, et al. Genetic variation of varicella-zoster virus: evidence for geographical separation of strains. J Med Virol. 2003; 70: S42-47.
  29. Kurukulasooriya GM, Thevanesam V, Agampodi SB, Abeykoon AM, Amarasinghe SP, Goonasekara KP. Susceptibility of New Entrant University Students in Sri Lanka to Varicella Zoster Infection. Asia Pac J Public Health.2009; 22: 219-224.
  30. Loparev VN, Gonzalez A, Deleon-Carnes M, Tipples G, Fickenscher H, Torfason EG, et al. Global identification of three major genotypes of varicella-zoster virus: longitudinal clustering and strategies for genotyping. J Virol. 2004; 78: 8349-8358.
  31. Springfeld C, Sauerbrei A, Filusch A, Konstandin M, Hartschuh W, Sauer P, et al. Fatal varicella in an immunocompromised adult associated with a European genotype E2 variant of varicella zoster virus. J Clin Virol. 2009; 44: 70-73.
  32. van der Werff Ten Bosch JE, Kollen WJ, Ball LM, Brinkman DM, Vossen AC, Lankester AC, et al. Atypical varicella zoster infection associated with hemophagocytic lymphohistiocytosis. Pediatr Blood Cancer. 2009; 53: 226-228.
  33. Quinlivan M, Sengupta N, Breuer J. A case of varicella caused by co-infection with two different genotypes of varicella-zoster virus. J Clin Virol. 2009; 44: 66-69.
  34. Loparev VN, Rubtcova EN, Bostik V, Tzaneva V, Sauerbrei A, Robo A, et al. Distribution of Varicella Zoster virus (VZV) wild type genotypes in northern and southern Europe: evidence for high conservation of circulating genotypes. Virol. 2009; 383: 216-225.
  35. Sauerbrei A, Wutzler P. Serological detection of specific IgG to varicella-zoster virus by novel ELISA based on viral glycoprotein antigen. Clin Lab. 2009; 55: 1-7.
  36. Knösel T, Schewe C, Petersen N, Dietel M, Petersen I. Prevalence of infectious pathogens in Crohn's disease. Pathol Res Pract. 2009; 205: 223-230.
  37. Kosa SC, Younge BR, Kumar N. Headaches due to giant cell arteritis following herpes zoster ophthalmicus in an elderly patient. Cephalalgia.2009; 19438922.
  38. Sáez-Llorens X, Yogev R, Arguedas A, Rodriguez A, Spigarelli MG, De León Castrejón T, et al. Pharmacokinetics and safety of famciclovir in children with herpes simplex or varicella-zoster virus infection. Antimicrob Agents Chemother. 2009; 53: 1912-1920.
  39. Peritz DC, Duncan C, Kurek K, Perez-Atayde AR, Lehmann LE. Visceral varicella zoster virus (VZV) after allogeneic hematopoietic stem cell transplant (HSCT) in pediatric patients with chronic graft-versus-host disease (cGVHD). J Pediatr Hematom Oncol. 2008; 30: 931-934.
  40. Siennicka J, Trzcińska A. Central nervous system viral infections-analysis of routine laboratory results. Med Dosw Mikrobiol. 2008; 60: 253-258.
  41. Tavazzi E, Minoli L, Ferrante P, Scagnelli P, Del Bue S, Romani A, et al. Varicella zoster virus meningo-encephalo-myelitis in an immunocompetent patient. Neurol Sci. 2008; 29: 279-283.
  42. Woolery WA. Herpes zoster virus vaccine. Geriatrics. 2008; 63: 6-9.
  43. Wutzler P. Herpes zoster and postherpetic neuralgia - prevention by vaccination? Dtsch Med Wochenschr. 2008; 134: S90-94.
  44. McGeoch DJ. Lineages of varicella-zoster virus. J Gen Virol. 2009; 90: 963-969.
  45. Onozawa M, Hashino S, Haseyama Y, Hirayama Y, Iizuka S, Ishida T, et al. Incidence and risk of postherpetic neuralgia after varicella-zoster virus infection in hematopoietic cell transplantation recipients: Hokkaido hematology study group. Biol Blood Marrow Transplant. 2009; 15: 724-729.
  46. Vandercam T, Hintzen RQ, de Boer JH, Van der Lelij A. Herpetic encephalitis is a risk factor for acute retinal necrosis. Neurology. 2008; 71: 1268-1274.
  47. Sutradhar SC, Wang WW, Schlienger K, Stek JE, Xu J, Chan IS, Silber JL. Comparison of the levels of immunogenicity and safety of Zostavax in adults 50 to 59 years old and in adults 60 years old or older. Clin Vaccine Immunol. 2009; 16: 646-652.
  48. Loparev VN, Rubtcova E, Seward J, Schmid DS, Levin M. DNA Sequence Variability in Isolates Recovered from Patients with Postvaccination Rash or Herpes Zoster Caused by Oka Varicella Vaccine. J Infect Dis. 2007; 195: 802-810.
  49. Carr MJ, McCormack GP, Crowley B. Genetic variation in clinical varicella-zoster virus isolates collected in Ireland between 2002 and 2003. J Med Virol. 2004; 73: 131-136.
  50. Liu M, Vafai N, Liu A, Hart J, Liu H, He J, et al. Stability of varicella-zoster virus open reading frame 63. Arch Virol. 2008; 153: 1943-1947.
  51. Davison AJ, Scott JE. The complete DNA sequence of varicella-zoster virus. J Gen Virol. 1986; 67: 1759-1816.
  52. Gomi Y, Sunamachi H, Mori Y, Nagaike K, Takahashi M, Yamanishi K. Comparison of the komplete DNA sequences of the Oka varicella vaccine and its parentel virus. J Virol. 2002; 76; 11447-11459.
  53. Loparev VN, McCaustland K, Holloway BP, Krause PR, Takayama M, Schmid DS. Rapid genotyping of varicella-zoster virus vaccine and wild-type strains with fluorophore-labeled hybridization probes. J Clin Microbiol. 2000; 38: 4315-4319.
  54. Loparev V, Martro E, Rubtcova E, Rodrigo C, Piette JC, Caumes E, et al. Toward universal varicella zoster virus genotyping: diversity of VZV strains from France and Spain. J Clin Microbiol. 2006; 45: 559-563.
  55. Norberg P, Liljeqvist JA, Bergström T, Sammons S, Schmid DS, Loparev VN. Complete-genome phylogenetic approach to varicella zoster virus evolution: genetic divergence and evidence for recombination. J Virol. 2006; 80: 9569-9576.
  56. Muir WB, Nichols R, Breuer J. Phylogenetic analysis of varicella-zoster virus: evidence of intercontinental spread of genotypes and recombination. J Virol. 2002; 76: 1971-1979.
  57. Muir WB, Nichols GP, Breuer J. Phylogenetic analysis of Genetic variation in clinical varicella-zoster virus: evidence of intercontinental spread of genotypes isolates collected in Ireland between 2002 and recombination. J Med Virol. 2004; 7673: 1971-9131.
  58. LaRussa P, Lungu O, Hardy I, Gershon A, Steinberg SP, Silverstein S. Restriction fragment length polymorphism of polymerase chain reaction products from vaccine and wild-type varicella-zoster virus isolates. J Virol. 1992; 66: 1016-1020.
  59. Campsall PA, Au NH, Prendiville JS, Speert DP, Tan R, Thomas EE. Detection and genotyping of varicella-zoster virus by TaqMan allelic discrimination real-time PCR. J Clin Microbiol. 2004; 42: 1409-1413.
  60. Nagel MA, Gilden D, Shade T, Gao B, Cohrs RJ. Rapid and sensitive detection of 68 unique varicella zoster virus gene transcripts in five multiplex reverse transcription-polymerase chain reactions. J Virol Methods. 2009; 157: 62-68.
  61. Faga B, Maury W, Bruckner DA, Grose C. Identification and mapping of single nucleotide polymorphisms in the varicella-zoster virus genome. Virology. 2001; 280: 1-6.
  62. Parker SP, Quinlivan M, Taha Y, Breuer J. Genotyping of varicella-zoster virus and the discrimination of Oka vaccine strains by TaqMan real-time PCR. J Clin Microbiol. 2006; 44: 3911- 3914.
  63. Li Y, Zhu B. Gnotyping of clinical varicella-zoster virus isolates collected from Yunnan in Southwestern China. Biomed Rep. 2016; 4: 209-214.
  64. Loparev VN, Argaw T, Krause PR, Takayama M, Schmid DS. Improved identification and differentiation of varicella-zoster virus (VZV) wild-type strains and an attenuated varicella vaccine strain using a VZV open reading frame 62-based PCR. J Clin Microbiol. 2000; 38: 3156-3160.
  65. Loparev VN, Rubtcova EN, Bostik V, Govil D, Birch CJ, Druce JD, et al. Identification of five major and two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand. J Virol. 2007; 81: 12758-12765.
  66. Liu J, Wang M, Gan L, Yang S, Chen J. Genotyping of clinical varicella-zoster virus isolates collected in China. J Clin Microbiol. 2009; 47: 1418-1423.
  67. Peters GA, Tyler SD, Grose C, Severini A, Gray MJ, Upton C, et al. A full-genome phylogenetic analysis of varicella-zoster virus reveals a novel origin of replication-based genotyping 88 scheme and evidence of recombination between major circulating genotypes. J Virol. 2006; 80: 9850-9860.
  68. Macneil A, Reynolds MG, Braden Z, Carroll DS, Bostik V, Karem K, et al. Transmission of atypical varicella-zoster virus infectious involving palm and sole manifestations in an area with monkeypox endemicity. Clin Infect Dis. 2009; 48: e6-8.
  69. f