Talita Helena Araújo de Oliveira1, Marconi Rego Barros Jr1, Daffany Luana dos Santos1, Ruany Cristyne de Oliveira Silva1, Bianca São Marcos1, Kamylla Conceição Gomes Nascimento1, Lígia Rosa Sales Leal1, Jacinto Costa da Silva Neto2, Anna Jéssica Duarte Silva1 and Antonio Carlos de Freitas1*
1Department of Genetics, Federal University of Pernambuco UFPE, Brazil 2Department of Embryology and Histology, Federal University of Pernambuco UFPE, BrazilFulltext PDF
High-risk Human papillomavirus (HPV) plays a key role in cervical cancer development due to its oncoprotein activities. The most frequent genotype in cervical lesions around the world is HPV-16, but other types are also founded, and the presence of multi-infection is associated with a higher risk of cervical cancer. E5 viral oncoprotein has a large range of tumorigenic attributes, including the modulation of microRNAs expression and previous in vitro studies have found an inverse relationship between E5 and microRNA-203, although no direct correlation was reported. Therefore, this study aimed to evaluate the profile of HPV infection and the possible correlation between E5 and microRNA-203 expression. Eighty-one fresh biopsies classified as normal tissue, cervical intraepithelial neoplasia grade (I, II, and III), and cancer were analyzed by qPCR. 83.95% of the samples were positive for HPV infection, and HPV-16 was the most prevalent, followed by HPV-31, HPV-58, HPV-18, and HPV-33. 29.41% of the samples were positive for more than one type (HPV-16 and HPV-31; HPV-16 and HPV58; HPV-31 and HPV-58; HPV-33 and HPV-58; HPV-18 and HPV-31; HPV-58 and HPV-18; HPV-16 and HPV-31 and HPV-18). We observed an increased expression of E5 in high-grade stages and cancer specimens, while microRNA-203 showed an opposite expression pattern from E5 mRNA, displaying reduced expression levels in cervical intraepithelial neoplasia III and cancer. These results help us to understand the HPV infection better, and even with no correlation, E5 may still alter miR-203 indirectly.
Human papillomavirus; Cervical cancer; Gene expression; E5 oncogene; microRNA
de Araújo Oliveira TH, Barros Jr MR, dos Santos DL, de Oliveira Silva RC, Marcos BS, Gomes Nascimento KC, et al. Expression of the Oncoprotein E5 from Human papillomavirus and miR- 203 in Pre-Cancer Lesions and Cervical Cancer. Clin Oncol. 2020; 5: 1737.