Leicester University, UKFulltext PDF
Bacteria have evolved different mechanisms of communicating with their environment and with other prokaryotic and eukaryotic cells. A secretion system identified recently is the Type VI Secretion System (T6SS) found in approximately 25% of gram-negative bacteria. The T6SS secretion apparatus is associated with the opportunistic gram-negative pathogen, Pseudomonas aeruginosa, which is known to cause Cystic Fibrosis (CF) and other life-threatening bacterial infections such as Pneumonia and Septicemia. Studies have demonstrated that T6SS in P. aeruginosacan penetrate the peptidoglycan cell wall of prokaryoticbacteria and insert two key effectors proteins Tse1 and Tse3.
The evolutionary adaption of T6SS secretion system gives the bacterium a unique survival advantage over other bacteria within the same niche. Although, the function, localization and mode of action of T6SS are being investigated, we do not fully understand the mechanism by which toxic proteins are delivered to neighboring prokaryotic cells.The study will investigate T6SS apparatus in P. aeruginosaby exploring the structure and identifying the functions of the three effector proteins Tse1-3 delivered to neighboring cells. It will explore the hypothesis that T6SS secretion system can be manipulated, through adaption, to deliver antibacterial drugs advantageous to the host and potentially alter disease outcome. Our research provides a platform to divulge, elucidate and manipulate a truly remarkable mechanism that could deliver a new treatment model for treating bacterial infection.
Khan R. Adapting T6SS Secretion Systems to Deliver Antibacterial Drugs to Eliminate Pseudomonas aeruginosa. Bioinform Int. 2020;1(1):1004..