Ann Short Rep | Volume 2, Issue 3 | Research Article | Open Access

MicroRNA-219c-5p May Participate in Bladder Fibrosis in Multiple Sclerosis Mice by Regulating Fibronectin-1

Bowen L1,2, Yafei D1, Peng L1, Wang T1, Siyuan H1, Zhankui J1,2, Chaohui G1,2 and Jinjian Y1,2*

1Department of Urology, Zhengzhou University First Affiliated Hospital, China
2Zhengzhou Institute of Urology, Zhengzhou University First Affiliated Hospital, China

*Correspondance to: Jinjian Y 

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Abstract

Background: We found that the bladder of the multiple sclerosis mice was significantly fibrosis. This study aimed to investigate the relationship between Fibronectin-1 (FN1) and bladder fibrosis, as well as microRNAs involved in the regulation of FN1. Methods: The degree of bladder smooth muscle fibrosis was observed by immunohistochemistry. And we used RT-qPCR (quantitative real-time polymerase chain reaction) and Western blot to identify the expression of FN1 in different grades of fibrosis bladder. It was found by bio information website analysis that miR-1a-3p, miR-219c-5p and miR-3572-3p may prevent inhibition of FN1 synthesis. Thus, miR-1a-3p, miR-219c-5p and miR-3572-3p were overexpressed or knocked down in bladder smooth muscle cells (BMSCs), and the respective transfection efficiency and FN1 knockdown efficiency were detected by RT-qPCR. We discovered that only overexpression and knockdown of miR-219c-5p met the expected results. The dual luciferase reporter assay was used to determine the targeting relationship between miR-219c-5p and FN1. Flow cytometry and Cell Counting Kit 8 (CCK8) experiments confirmed that miR-219c-5p reduced FN1 and affected the biological activity of smooth muscle cells. Results: As bladder fibrosis worsens, the expression of FN1 is raised, while the expression levels of miR-1a-3p, miR-219c-5p, and miR-3572-3P are decreased. The results of RT-qPCR after transfection showed that only miR-219c-5p was the best to regulate FN1. The results of the dual luciferase reporter gene indicated that miR-219c-5p is targeted to bind to FN1. CCK8 assay and cell cycle assay showed that overexpression of miR-219c-5p inhibited the proliferation of BMSCs, while knockdown of miR-219c-5p promoted the proliferation of BMSCs. Apoptosis assay showed that overexpression of miR-219c-5p promoted apoptosis, while knockdown of miR-219c-5p inhibited apoptosis of BMSCs. Conclusion: Our findings indicate that up-regulation of FN1 and down-regulation of miR-219c-5p play an important role in the development of bladder fibrosis. And miR-219c-5p may be involved in bladder fibrosis by targeting FN1 expression.

Keywords:

Multiple sclerosis; Experimental autoimmune encephalomyelitis; Bladder fibrosis; Fibronectin-1; microRNA-219c-5p

Citation:

Bowen L, Yafei D, Peng L, Wang T, Siyuan H, Zhankui J, et al. MicroRNA- 219c-5p May Participate in Bladder Fibrosis in Multiple Sclerosis Mice by Regulating Fibronectin-1. Ann Short Reports. 2019; 2: 1044.

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