Donghui Z, Qian F and Jinghua L*
Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, and Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing, ChinaFulltext PDF
To elucidate the protective effects of Theaflavin (TF1) on myocardial Ischemia-Reperfusion (I/R) injury. A rat myocardial I/R injury model was prepared by constriction of the anterior descending branch of the coronary artery and recanalization. Intracellular contents of reactive oxygen species and reactive nitrogen species were detected by flow cytometry and ELISA. Protein expression levels of autophagy-related proteins were determined by western blotting. Application of TF1 20 μmol/L inhibited the changes in heart rate, heart weight/body weight ratio, and left ventricular peripheral thickness observed in the I/R group. In particular, the change in the infarct area was significantly reduced. Compared with the I/R group, the TF1 group had slightly disorganized myocardial fibers with better morphology, relatively clear nuclei, cell transverse stria, and intercalary discs, and only small amounts of necrotic areas and inflammatory cell infiltration. The ROS increase in the I/R group was decreased by the application of TF1. Furthermore, treatment with TF1 reduced that the iNOS activity, NOX level, and nitrotyrosine content observed in the I/R group. The apoptosis index of myocardial cells in the I/R group was significantly alleviated by TF1 from 38.4% ± 3.4% to 21.3% ± 2.7%. TF1 also inhibited the peak time of the intracellular calcium transient as well as the 90% decay time. Protein expression of LC3-II was markedly reduced by TF1. The increases in Beclin-1 and Vps34 protein expression observed in the I/R group were significantly inhibited by TF1. TF1 plays an important role in autophagy regulation through the up-regulation of Beclin-1/Vps34 protein expression.
Theaflavin; Cardiac ischemia-reperfusion; Autophagy; Beclin-1/Vps34 complex
Donghui Z, Qian F, Jinghua L. Effects of Theaflavin on Beclin-1/Vps34 Complex Regulation during Autophagy in Myocardial Ischemia-Reperfusion Injury in Rats. Ann Pharmacol Pharm. 2020;5(4):1186.