Mervat Radwan1*, Rabab Elzoghby R2, Aziza Amin3, Rania Abo-Sakaya4 and Ahlam Hamouda F5
1Department of Infectious Disease, Benha University, Egypt 2Department of Pharmacology, New Vally University, Egypt 3Department of Pathology, Benha University, Egypt 4Department of Infectious Disease, Benha University, Egypt 5Department of Forensic Medicine and Toxicology, Benha University, EgyptFulltext PDF
This study was carried out to investigate the effect of experimental infection by Escherichia coli O157 on some biochemical parameters and histopathological changes in rats and current methods of treatment to reach this objective (number of rats 25 divided in three groups) all groups were housed under same conditions of water, food, temperature, light and humidity (one week for acclimatization) control groups which divided into two groups (Group I was served as a control negative. Group II served as control positive group infected with E. coli O157 2 ml of (1 × 109) colony forming unit/ml at the 7th day of experiment without treatment. Probiotics groups that divided into Group III: Rats received lactobacillus as probiotic by dose of 3.6 × 1021 dissolved in distalled water orally by stomach tube all over the time of experiment (14 days) and infected with E. coli O157 2 ml of (1 × 109) colony forming unit/ml at 7th day of experiment and treated with ciprofloxacin by dose of 14.4 mg/Kg.b.wt once daily by intraperitoneal rout at three days after infection for five successive days, Group IV: Rats received Lactobacillus as probiotic by dose of 3.6 × 1021 dissolved in distalled water orally by stomach tube all over the time of experiment (14 days) and infected with E. coli O157 2 ml of (1 × 109 colony forming unit/ml) at 7th day of experiment. Group V: Rats infected with E. coli O157 2 ml of (1 × 109) colony forming unit/ml at 7th day of the experiment and treated with ciprofloxacin by dose of 14.4 mg/Kg.b.wt once daily by intraperitoneal injection at three days post infection and for five successive days. After five days from infection, blood samples were collected from all groups from retro-orbital sinus by using of capillary tube to measure various biochemical changes, which affected by infection in all groups respect to control groups as well as samples from different organs (liver, kidneys, lung, heart, spleen, brain, testes, and intestine) were taken for histopathology examination. The biochemical and histopathological changes in all groups were recorded regarding to the biochemical changes, histopathological and fertility examination, the results revealed that significant different between infected and treated groups in comparison to negative control one. These results demonstrate that the administration of the probiotic Lactobacillus debruckii and Lactobacillus fernentum in combination with ciprofloxacin to rats can reduce the severity of E. coli O157:H7 infection, reduce toxicity and histopathological alterations in different organs. Consequently, this reduction may be associated with enhancement of liver and kidney function and immune responses.
Experimental infection; E. coli O157 toxicity; Histopathology; Ciprofloxacin and probiotic therapy
Radwan M, Rashed RHamoda AF, Amin A, Sakaya RB. Experimental Infection with E. coli O157 in Rats and Its Toxic Effect, Biochemical and Histopathological Changes with Referee to Modern Therapy. Ann Microbiol Immunol. 2021; 4(1): 1024..